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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-6-8
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pubmed:abstractText |
It has been known that the expression of inducible nitric oxide synthase (iNOS) is up-regulated during hepatic regeneration. The present study characterized the molecular mechanisms involved in the transcriptional activation of iNOS gene by using the serum after partial hepatectomy (post-PH serum) in vitro. The post-PH serum rapidly induced iNOS mRNA expression, which was blocked by anti-tumor necrosis factor-alpha (TNF-alpha) antibody in BNL CL.2 cells, murine embryonic liver cell line. In addition, EMSAs using a NF-kappa B-specific oligomer showed that the up-regulated iNOS mRNA expression in cells treated with post-PH serum correlated with transient activation of NF-kappa B complex (p50/p65 heterodimer). Transient transfection of BNL CL.2 cells with iNOS promoter linked to a CAT reporter gene showed the transcriptional activation of iNOS promoter by post-PH serum. Furthermore, site-directed mutational analysis of the two NF-kappa B sites individually or in combination revealed that iNOS expression by post-PH serum is regulated by the downstream NF-kappa B site, but not by upstream NF-kappa B site. Taken together, these results suggest that the downstream NF-kappa B site acts as an essential component for the iNOS expression by post-PH serum during hepatic regeneration.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
607-13
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11396944-Animals,
pubmed-meshheading:11396944-Antibodies,
pubmed-meshheading:11396944-Blood,
pubmed-meshheading:11396944-Cell Line,
pubmed-meshheading:11396944-Culture Media,
pubmed-meshheading:11396944-Hepatectomy,
pubmed-meshheading:11396944-Kinetics,
pubmed-meshheading:11396944-Liver Regeneration,
pubmed-meshheading:11396944-Mice,
pubmed-meshheading:11396944-NF-kappa B,
pubmed-meshheading:11396944-Nitric Oxide Synthase,
pubmed-meshheading:11396944-Nitric Oxide Synthase Type II,
pubmed-meshheading:11396944-Promoter Regions, Genetic,
pubmed-meshheading:11396944-RNA, Messenger,
pubmed-meshheading:11396944-Rats,
pubmed-meshheading:11396944-Rats, Sprague-Dawley,
pubmed-meshheading:11396944-Response Elements,
pubmed-meshheading:11396944-Transcriptional Activation,
pubmed-meshheading:11396944-Tumor Necrosis Factor-alpha
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pubmed:year |
2001
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pubmed:articleTitle |
Serum after partial hepatectomy stimulates iNOS gene transcription via downstream NF-kappa B site.
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pubmed:affiliation |
Department of Pharmacology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-gu, Taejon, Korea, 301-131. gmhur@hanbat.chungnam.ac.kr
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pubmed:publicationType |
Journal Article
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