Linked Life Data

11396133

Source:http://linkedlifedata.com/resource/pubmed/id/11396133

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2A
pubmed:dateCreated
2001-6-8
pubmed:abstractText
PSK is a plant polysaccharide widely used for cancer immunotherapy in Japan and other Asian countries. It is considered that its antitumor effect is derived from its immunomodulating activity on the tumor-bearing host. The present study was designed to assess the direct action of PSK on in vitro proliferation and invasion of human KATO-3 gastric and Colo205 colonic cancer cell lines, and the expression of surface molecules such as HLA and adhesion molecules on these cells. The in vitro growth of KATO-3 cells was significantly inhibited by 100 micrograms/ml of PSK 48 hrs after culture initiation, and that of Colo205 was significantly inhibited by 10 and 100 micrograms/ml of PSK 24 hrs after culture initiation. The effect of PSK on the in vitro invasion of the tumor cells, assessed with a Matrigel invasion chamber, revealed that invasion of KATO-3 and Colo205 cells was inhibited by more than 10 micrograms/ml and more than 5 micrograms/ml of PSK, respectively. KATO-3 cells expressed HLA-ABC, HLA-A2/A28, HLA-DR very weakly, at almost baseline levels, but HLA-B27, B2-microglobulin and HLA-DQ were expressed at various levels. After treatment of KATO-3 cells with PSK, the expression of HLA-B27 and beta 2-microglobulin was significantly enhanced. Colo205 cells expressed all class-I antigens tested in this study at different levels, but class-II antigens at almost baseline levels. PSK also enhanced the expression of class-I antigens on Colo205 cells. ICAM-1 was expressed on KATO-3, but not on Colo205. The expression of ICAM-1 was enhanced to a greater extent by treatment with 10 micrograms/ml than with 100 micrograms/ml of PSK. Adenocarcinoma antigen AC-81 was strongly expressed on both cell lines, but PSK-treatment significantly enhanced its expression. These results suggested that enhancement of HLA class-I expression on tumor cells after PSK treatment may be one of the mechanisms responsible for the induction of anti-tumor immunity by PSK.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0250-7005
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1007-13
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11396133-Antineoplastic Agents, Phytogenic, pubmed-meshheading:11396133-Cell Division, pubmed-meshheading:11396133-Cell Membrane, pubmed-meshheading:11396133-Colonic Neoplasms, pubmed-meshheading:11396133-HLA Antigens, pubmed-meshheading:11396133-HLA-DQ Antigens, pubmed-meshheading:11396133-HLA-DR Antigens, pubmed-meshheading:11396133-Histocompatibility Antigens Class I, pubmed-meshheading:11396133-Humans, pubmed-meshheading:11396133-Immunologic Factors, pubmed-meshheading:11396133-Intercellular Adhesion Molecule-1, pubmed-meshheading:11396133-Lymphocyte Function-Associated Antigen-1, pubmed-meshheading:11396133-Neoplasm Invasiveness, pubmed-meshheading:11396133-Proteoglycans, pubmed-meshheading:11396133-Stomach Neoplasms, pubmed-meshheading:11396133-Tumor Cells, Cultured
pubmed:articleTitle
Plant polysaccharide PSK: cytostatic effects on growth and invasion; modulating effect on the expression of HLA and adhesion molecules on human gastric and colonic tumor cell surface.
pubmed:affiliation
First Department of Surgery, Shimane Medical University, 89-1, Enya-cho, Izumo, Shimane 693-8501, Japan.
pubmed:publicationType
Journal Article