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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-6-7
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pubmed:abstractText |
Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pH(i)) and Ca2+ ([Ca2+]i) it induces in sperm. We observed significant time delays for the increase in pH(i) and [Ca2+]i induced by 200 nM speract (69 and 190 ms, respectively). Also, we found that the receptor undergoes a pH(i)-dependent affinity change at around 129 ms. These time delays probably reflect biochemical processes underlying each sperm response to speract that circumscribe the time sequence of the signaling events.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
531-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11394914-Animals,
pubmed-meshheading:11394914-Calcium,
pubmed-meshheading:11394914-Chemotaxis,
pubmed-meshheading:11394914-Fluorescent Dyes,
pubmed-meshheading:11394914-Fluorometry,
pubmed-meshheading:11394914-Hydrogen-Ion Concentration,
pubmed-meshheading:11394914-Intracellular Fluid,
pubmed-meshheading:11394914-Male,
pubmed-meshheading:11394914-Oligopeptides,
pubmed-meshheading:11394914-Protein Binding,
pubmed-meshheading:11394914-Reaction Time,
pubmed-meshheading:11394914-Receptors, Cell Surface,
pubmed-meshheading:11394914-Sea Urchins,
pubmed-meshheading:11394914-Signal Transduction,
pubmed-meshheading:11394914-Spermatozoa
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pubmed:year |
2001
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pubmed:articleTitle |
Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry.
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pubmed:affiliation |
Departamento de Genética y Fisiología Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, 62250, México.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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