Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-6-7
pubmed:abstractText
Here we report the expression, in the human ocular ciliary epithelium and in a human nonpigmented (NPE) ciliary epithelial cell line, of genes usually restricted to cone and rod photoreceptor cells of the retina. By RT-PCR and DNA sequencing we identified the expression of rhodopsin and components linked to its deactivation, including rhodopsin kinase, recoverin, and visual arrestin. We also detected the expression of transducin (T-alpha), phosphodiesterase (PDE-alpha), and cGMP-gated channel alpha-subunits. Cultured NPE cells responded to treatment with phorbol ester by enhancing the expression of rhodopsin mRNA three- to fourfold. Indirect immunofluorescence of the intact ciliary epithelium with monoclonal antibodies (MAbs) against rhodopsin, rhodopsin kinase, and visual arrestin revealed labeling preferentially restricted to the NPE cells. Furthermore, Western blot analysis of whole lysates from the pars plicata region of the human ciliary epithelium with MAbs demonstrated immunochemical cross-reactivity with proteins of molecular mass similar to rhodopsin (36 kDa), rhodopsin kinase (64 to 66 kDa), and arrestin (48-52 kDa) from the human retina. These results provide the first molecular evidence that components of a non-visual phototransduction pathway are expressed in the human ocular NPE ciliary epithelium, which may be linked to circadian entrainment tasks.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Arrestin, http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic Nucleotide-Gated Cation..., http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins, http://linkedlifedata.com/resource/pubmed/chemical/G-Protein-Coupled Receptor Kinase 1, http://linkedlifedata.com/resource/pubmed/chemical/GRK1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Hippocalcin, http://linkedlifedata.com/resource/pubmed/chemical/Ion Channels, http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoric Diester Hydrolases, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits, http://linkedlifedata.com/resource/pubmed/chemical/RCVRN protein, human, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Recoverin, http://linkedlifedata.com/resource/pubmed/chemical/Rhodopsin, http://linkedlifedata.com/resource/pubmed/chemical/Transducin
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2001 Academic Press.
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
317-25
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11394879-Animals, pubmed-meshheading:11394879-Antibodies, Monoclonal, pubmed-meshheading:11394879-Arrestin, pubmed-meshheading:11394879-Blotting, Western, pubmed-meshheading:11394879-Calcium-Binding Proteins, pubmed-meshheading:11394879-Cattle, pubmed-meshheading:11394879-Cell Line, pubmed-meshheading:11394879-Ciliary Body, pubmed-meshheading:11394879-Cyclic Nucleotide-Gated Cation Channels, pubmed-meshheading:11394879-Epithelial Cells, pubmed-meshheading:11394879-Eye Proteins, pubmed-meshheading:11394879-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:11394879-G-Protein-Coupled Receptor Kinase 1, pubmed-meshheading:11394879-Hippocalcin, pubmed-meshheading:11394879-Humans, pubmed-meshheading:11394879-Immunoblotting, pubmed-meshheading:11394879-Immunohistochemistry, pubmed-meshheading:11394879-Ion Channels, pubmed-meshheading:11394879-Light Signal Transduction, pubmed-meshheading:11394879-Lipoproteins, pubmed-meshheading:11394879-Nerve Tissue Proteins, pubmed-meshheading:11394879-Phosphoric Diester Hydrolases, pubmed-meshheading:11394879-Protein Kinases, pubmed-meshheading:11394879-Protein Subunits, pubmed-meshheading:11394879-RNA, Messenger, pubmed-meshheading:11394879-Recoverin, pubmed-meshheading:11394879-Retina, pubmed-meshheading:11394879-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11394879-Rhodopsin, pubmed-meshheading:11394879-Sequence Analysis, DNA, pubmed-meshheading:11394879-Transducin
pubmed:year
2001
pubmed:articleTitle
Molecular evidence that human ocular ciliary epithelium expresses components involved in phototransduction.
pubmed:affiliation
Department of Ophthalmology and Visual Science, Yale University School of Medicine, 330 Cedar Street, New Haven, Connecticut 06510, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't