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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22-23
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pubmed:dateCreated |
2001-6-5
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pubmed:abstractText |
Many different G protein-coupled receptors modulate the activity of Ca2+ and K+ channels in a variety of neuronal types. There are five known subtypes (M1-M5) of muscarinic acetylcholine receptors. Knockout mice lacking the M1, M2, or M4 subtypes are studied to determine which receptors mediate modulation of voltage-gated Ca2+ channels in mouse sympathetic neurons. In these cells, muscarinic agonists modulate N- and L-type Ca2+ channels and the M-type K+ channel through two distinct, G-protein mediated pathways. The fast and voltage-dependent pathway is lacking in the M2 receptor knockout mice. The slow and voltage-independent pathway is absent in the M1 receptor knockout mice. Neither pathway is affected in the M4 receptor knockout mice. Muscarinic modulation of the M current is absent in the M1 receptor knockout mice, and can be reconstituted in a heterologous expression system using cloned channels and M1 receptors. Our results using knockout mice are compared with pharmacological data in the rat.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2481-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11392616-Animals,
pubmed-meshheading:11392616-Calcium Channels,
pubmed-meshheading:11392616-Electrophysiology,
pubmed-meshheading:11392616-Enzyme Inhibitors,
pubmed-meshheading:11392616-Ethylmaleimide,
pubmed-meshheading:11392616-GTP-Binding Proteins,
pubmed-meshheading:11392616-Mice,
pubmed-meshheading:11392616-Mice, Knockout,
pubmed-meshheading:11392616-Muscarinic Agonists,
pubmed-meshheading:11392616-Neurons,
pubmed-meshheading:11392616-Oxotremorine,
pubmed-meshheading:11392616-Potassium Channels,
pubmed-meshheading:11392616-Protein Isoforms,
pubmed-meshheading:11392616-Rats,
pubmed-meshheading:11392616-Receptors, Muscarinic,
pubmed-meshheading:11392616-Signal Transduction,
pubmed-meshheading:11392616-Superior Cervical Ganglion,
pubmed-meshheading:11392616-Time Factors
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pubmed:year |
2001
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pubmed:articleTitle |
Identification of subtypes of muscarinic receptors that regulate Ca2+ and K+ channel activity in sympathetic neurons.
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pubmed:affiliation |
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195-7290, USA. shapirom@uthscsa.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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