Source:http://linkedlifedata.com/resource/pubmed/id/11391618
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-6-6
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| pubmed:abstractText |
8-Cl-cyclic adenosine monophosphate (8-Cl-cAMP) has been known to induce growth inhibition and differentiation in a variety of cancer cells by differential modulation of protein kinase A isozymes. To understand the anticancer activity of 8-Cl-cAMP further, we investigated the effect of 8-Cl-cAMP on apoptosis in human cancer cells. Most of the tested human cancer cells exhibited apoptosis upon treatment with 8-Cl-cAMP, albeit with different sensitivity. Among them, SH-SY5Y neuroblastoma cells and HL60 leukemic cells showed the most extensive apoptosis. The effect of 8-Cl-cAMP was not reproduced by other cAMP analogues or cAMP-elevating agents, showing that the effect of 8-Cl-cAMP was not caused by simple activation of protein kinase A (PKA). However, competition experiments showed that the binding of 8-Cl-cAMP to the cAMP receptor was essential for the induction of apoptosis. After the treatment of 8-Cl-cAMP, cells initially accumulated at the S and G2/M phases of the cell cycle and then apoptosis began to occur among the population of cells at the S/G2/M cell cycle phases, indicating that the 8-Cl-cAMP-induced apoptosis is closely related to cell cycle control. In support of this assumption, 8-Cl-cAMP-induced apoptosis was blocked by concomitant treatment with mimosine, which blocks the cell cycle at early S phase. Interestingly, 8-Cl-cAMP did not induce apoptosis in primary cultured normal cells and non-transformed cell lines, showing that 8-Cl-cAMP-induced apoptosis is specific to transformed cells. Taken together, our results show that the induction of apoptosis is one of the mechanisms through which 8-Cl-cAMP exerts anticancer activity.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/8-chloro-cyclic adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cyclic AMP
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0020-7136
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
93
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
33-41
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11391618-3T3 Cells,
pubmed-meshheading:11391618-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:11391618-Animals,
pubmed-meshheading:11391618-Antineoplastic Agents,
pubmed-meshheading:11391618-Apoptosis,
pubmed-meshheading:11391618-Breast Neoplasms,
pubmed-meshheading:11391618-CHO Cells,
pubmed-meshheading:11391618-Cell Cycle,
pubmed-meshheading:11391618-Cricetinae,
pubmed-meshheading:11391618-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:11391618-Female,
pubmed-meshheading:11391618-G2 Phase,
pubmed-meshheading:11391618-HL-60 Cells,
pubmed-meshheading:11391618-HeLa Cells,
pubmed-meshheading:11391618-Humans,
pubmed-meshheading:11391618-K562 Cells,
pubmed-meshheading:11391618-Mice,
pubmed-meshheading:11391618-Mitosis,
pubmed-meshheading:11391618-Neuroblastoma,
pubmed-meshheading:11391618-Ovarian Neoplasms,
pubmed-meshheading:11391618-Receptors, Cyclic AMP,
pubmed-meshheading:11391618-S Phase,
pubmed-meshheading:11391618-Tumor Cells, Cultured
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| pubmed:year |
2001
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| pubmed:articleTitle |
8-Cl-cAMP induces cell cycle-specific apoptosis in human cancer cells.
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| pubmed:affiliation |
School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, Republic of Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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