Linked Life Data

11389758

Source:http://linkedlifedata.com/resource/pubmed/id/11389758

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-6-6
pubmed:databankReference
pubmed:abstractText
Cytoplasmic male sterility (CMS) in higher plants has been mainly studied in cultivated species. In most cases, pollen abortion is linked to the presence of an additional mitochondrial polypeptide leading to organelle dysfunction in reproductive tissues. In wild beet, both CMS and hermaphrodite plants coexist in natural populations. The G cytoplasm is widely distributed along the Western European coast, and previous genetic studies have demonstrated that this cytoplasm confers male sterility in beet. In the present study, we have identified two mutations of G mitochondrial genes, each of which results in the production of a respiratory chain complex subunit with an altered molecular weight; the NAD9 subunit has a C-terminal extension while the COX2 subunit has a truncated C-terminus. NADH dehydrogenase activity was unchanged in leaves, but cytochrome c oxidase activity was reduced by 50%. Moreover, Western blot analyses revealed that alternative oxidase was more abundant in male sterile G plants than in a fertile control (Nv), suggesting that this alternative pathway might compensate for the cytochrome c oxidase deficiency. Implications of respiratory chain changes and a putative link with CMS are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
171-80
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:11389758-Amino Acid Sequence, pubmed-meshheading:11389758-Base Sequence, pubmed-meshheading:11389758-Blotting, Northern, pubmed-meshheading:11389758-Blotting, Southern, pubmed-meshheading:11389758-Blotting, Western, pubmed-meshheading:11389758-Chenopodiaceae, pubmed-meshheading:11389758-Cyclooxygenase 2, pubmed-meshheading:11389758-Cytoplasm, pubmed-meshheading:11389758-Electron Transport, pubmed-meshheading:11389758-Electron Transport Complex I, pubmed-meshheading:11389758-Electron Transport Complex IV, pubmed-meshheading:11389758-Fertility, pubmed-meshheading:11389758-Gene Expression Regulation, Plant, pubmed-meshheading:11389758-Genome, Plant, pubmed-meshheading:11389758-Isoenzymes, pubmed-meshheading:11389758-Mitochondria, pubmed-meshheading:11389758-Mitochondrial Proteins, pubmed-meshheading:11389758-Molecular Sequence Data, pubmed-meshheading:11389758-Molecular Weight, pubmed-meshheading:11389758-Mutation, pubmed-meshheading:11389758-NADH, NADPH Oxidoreductases, pubmed-meshheading:11389758-Oxidoreductases, pubmed-meshheading:11389758-Plant Proteins, pubmed-meshheading:11389758-Pollen, pubmed-meshheading:11389758-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:11389758-Sequence Analysis
pubmed:year
2001
pubmed:articleTitle
The male sterile G cytoplasm of wild beet displays modified mitochondrial respiratory complexes.
pubmed:affiliation
Laboratoire de Génétique et Evolution des Populations Végétales, UPRESA-CNRS 8016, FR CNRS 1818, Université de Lille I, F-59655 Villeneuve d'Ascq cedex, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't