|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2001-6-6
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
The steady-state level of transcripts coding for the pyrroline-5-carboxylate reductase of Arabidopsis (At-P5R) increased under salt and heat stress, mainly because of an enhanced mRNA stability. However, the At-P5R protein level was not induced, and its translation was inhibited at initiation stage and probably also at later stages. Replacement of the 5' untranslated region (5'UTR) and beta-glucuronidase (gus) fusion analysis revealed that the first 92 bp region of the At-P5R 5'UTR was sufficient to mediate transcript stabilization and translation inhibition during salt and heat stresses. Furthermore, the first 92 bp region of the At-P5R 5'UTR was also involved in transcription efficiency in a promoter-dependent manner. The results demonstrated that the stress regulation of At-P5R is complex and involves the 5'UTR which acts at three levels, partly in opposing directions.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronidase,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrroline Carboxylate Reductases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/RNA primers,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/delta-1-pyrroline-5-carboxylate...
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0960-7412
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
26
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
157-69
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:11389757-5' Untranslated Regions,
pubmed-meshheading:11389757-Arabidopsis,
pubmed-meshheading:11389757-Base Sequence,
pubmed-meshheading:11389757-Blotting, Northern,
pubmed-meshheading:11389757-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11389757-Gene Expression Regulation, Plant,
pubmed-meshheading:11389757-Glucuronidase,
pubmed-meshheading:11389757-Hot Temperature,
pubmed-meshheading:11389757-Molecular Sequence Data,
pubmed-meshheading:11389757-Plant Proteins,
pubmed-meshheading:11389757-Polyribosomes,
pubmed-meshheading:11389757-Promoter Regions, Genetic,
pubmed-meshheading:11389757-Protein Biosynthesis,
pubmed-meshheading:11389757-Pyrroline Carboxylate Reductases,
pubmed-meshheading:11389757-RNA,
pubmed-meshheading:11389757-RNA, Messenger,
pubmed-meshheading:11389757-RNA, Plant,
pubmed-meshheading:11389757-RNA Processing, Post-Transcriptional,
pubmed-meshheading:11389757-Recombinant Fusion Proteins,
pubmed-meshheading:11389757-Sodium Chloride,
pubmed-meshheading:11389757-Transcription, Genetic,
pubmed-meshheading:11389757-Transcription Factors
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
The 5' untranslated region of the At-P5R gene is involved in both transcriptional and post-transcriptional regulation.
|
|
pubmed:affiliation |
Vakgroep Moleculaire Genetica & Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
