Linked Life Data

11389203

Source:http://linkedlifedata.com/resource/pubmed/id/11389203

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 2
pubmed:dateCreated
2001-6-4
pubmed:abstractText
1. The role of intracellular Ca(2+) mobilization in the mechanism of increased endothelial permeability was studied. Human umbilical vein endothelial cells (HUVECs) were exposed to thapsigargin or thrombin at concentrations that resulted in similar increases in intracellular Ca(2+) concentration ([Ca(2+)](i)). The rise in [Ca(2+)](i) in both cases was due to release of Ca(2+) from intracellular stores and influx of extracellular Ca(2+). 2. Both agents decreased endothelial cell monolayer electrical resistance (a measure of endothelial cell shape change) and increased transendothelial (125)I-albumin permeability. Thapsigargin induced activation of PKCalpha and discontinuities in VE-cadherin junctions without formation of actin stress fibres. Thrombin also induced PKCalpha activation and similar alterations in VE-cadherin junctions, but in association with actin stress fibre formation. 3. Thapsigargin failed to promote phosphorylation of the 20 kDa myosin light chain (MLC(20)), whereas thrombin induced MLC(20) phosphorylation consistent with formation of actin stress fibres. 4. Calphostin C pretreatment prevented the disruption of VE-cadherin junctions and the decrease in transendothelial electrical resistance caused by both agents. Thus, the increased [Ca(2+)](i) elicited by thapsigargin and thrombin may activate a calphostin C-sensitive PKC pathway that signals VE-cadherin junctional disassembly and increased endothelial permeability. 5. Results suggest a critical role for Ca(2+) signalling and activation of PKCalpha in mediating the disruption of VE-cadherin junctions, and thereby in the mechanism of increased endothelial permeability.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-10449777, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-10861009, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-11003570, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-11076812, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-1332502, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-1514647, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-1518814, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-1631567, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-2296576, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-2347922, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-2708261, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-7622562, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-7698986, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-7775594, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-7943249, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-8360259, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-8383691, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-8613524, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-8791416, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-8903311, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-9038902, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-9374664, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-9725917, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-9831706, http://linkedlifedata.com/resource/pubmed/commentcorrection/11389203-9886918
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Albumins, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Cadherins, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens, http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Desmoplakins, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Hemostatics, http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/Myosin Light Chains, http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes, http://linkedlifedata.com/resource/pubmed/chemical/PRKCA protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate, http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin, http://linkedlifedata.com/resource/pubmed/chemical/Thrombin, http://linkedlifedata.com/resource/pubmed/chemical/cadherin 5, http://linkedlifedata.com/resource/pubmed/chemical/calphostin C
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
533
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
433-45
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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