| pubmed-article:11388802 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11388802 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:11388802 | lifeskim:mentions | umls-concept:C0017776 | lld:lifeskim |
| pubmed-article:11388802 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:11388802 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:11388802 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:11388802 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:11388802 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11388802 | pubmed:dateCreated | 2001-6-4 | lld:pubmed |
| pubmed-article:11388802 | pubmed:abstractText | A strong and constitutive expression vector of Escherichia coli beta-glucuronidase with the isocitrate dehydrogenase promoter has been developed for producing a large amount of recombinant protein. More than 95% pure enzyme was obtained by a four step purification procedure-ammonium sulfate precipitation, DEAE ion-exchange chromatography, Superose 12 gel filtration, and hydroxyapatite steric ion-exchange chromatography. The overexpressed gene can produce 23 mg of pure enzyme from one liter of bacterial culture. | lld:pubmed |
| pubmed-article:11388802 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11388802 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11388802 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11388802 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11388802 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11388802 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11388802 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11388802 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11388802 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:11388802 | pubmed:issn | 1046-5928 | lld:pubmed |
| pubmed-article:11388802 | pubmed:author | pubmed-author:ChenRR | lld:pubmed |
| pubmed-article:11388802 | pubmed:author | pubmed-author:DelbaereL TLT | lld:pubmed |
| pubmed-article:11388802 | pubmed:author | pubmed-author:AichSS | lld:pubmed |
| pubmed-article:11388802 | pubmed:copyrightInfo | Copyright 2001 Academic Press. | lld:pubmed |
| pubmed-article:11388802 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11388802 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:11388802 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11388802 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11388802 | pubmed:pagination | 75-81 | lld:pubmed |
| pubmed-article:11388802 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
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| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:meshHeading | pubmed-meshheading:11388802... | lld:pubmed |
| pubmed-article:11388802 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11388802 | pubmed:articleTitle | Expression and purification of Escherichia coli beta-glucuronidase. | lld:pubmed |
| pubmed-article:11388802 | pubmed:affiliation | Department of Biochemistry, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada. | lld:pubmed |
| pubmed-article:11388802 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11388802 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:946149 | entrezgene:pubmed | pubmed-article:11388802 | lld:entrezgene |
