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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-6-4
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pubmed:abstractText |
A strong and constitutive expression vector of Escherichia coli beta-glucuronidase with the isocitrate dehydrogenase promoter has been developed for producing a large amount of recombinant protein. More than 95% pure enzyme was obtained by a four step purification procedure-ammonium sulfate precipitation, DEAE ion-exchange chromatography, Superose 12 gel filtration, and hydroxyapatite steric ion-exchange chromatography. The overexpressed gene can produce 23 mg of pure enzyme from one liter of bacterial culture.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1046-5928
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-81
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11388802-Chemical Precipitation,
pubmed-meshheading:11388802-Chromatography, Gel,
pubmed-meshheading:11388802-Chromatography, Ion Exchange,
pubmed-meshheading:11388802-DNA, Complementary,
pubmed-meshheading:11388802-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:11388802-Enzyme Stability,
pubmed-meshheading:11388802-Escherichia coli,
pubmed-meshheading:11388802-Genetic Vectors,
pubmed-meshheading:11388802-Glucuronidase,
pubmed-meshheading:11388802-Hydrogen-Ion Concentration,
pubmed-meshheading:11388802-Isocitrate Dehydrogenase,
pubmed-meshheading:11388802-Kinetics,
pubmed-meshheading:11388802-Mutagenesis, Site-Directed,
pubmed-meshheading:11388802-Polymerase Chain Reaction,
pubmed-meshheading:11388802-Promoter Regions, Genetic,
pubmed-meshheading:11388802-Recombinant Proteins,
pubmed-meshheading:11388802-Restriction Mapping,
pubmed-meshheading:11388802-Sequence Analysis, DNA
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pubmed:year |
2001
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pubmed:articleTitle |
Expression and purification of Escherichia coli beta-glucuronidase.
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pubmed:affiliation |
Department of Biochemistry, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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