| pubmed-article:11387317 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0016192 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0036113 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0282560 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0010851 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0540333 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:11387317 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11387317 | pubmed:issue | 32 | lld:pubmed |
| pubmed-article:11387317 | pubmed:dateCreated | 2001-8-6 | lld:pubmed |
| pubmed-article:11387317 | pubmed:abstractText | Antimicrobial peptides are crucial for host defense at mucosal surfaces. Bacterial factors responsible for induction of human beta-defensin-2 (hBD-2) mRNA expression in Caco-2 human carcinoma cells were determined. Salmonella enteritidis, Salmonella typhimurium, Salmonella typhi, Salmonella dublin, and culture supernatants of these strains induced hBD-2 mRNA expression in Caco-2 human carcinoma cells. Using luciferase as a reporter gene for a approximately 2.1-kilobase pair hBD-2 promoter, the hBD-2-inducing factor in culture supernatant of S. enteritidis was isolated. The supernatant factor was heat-stable and proteinase-sensitive. After purification by anion exchange and gel filtration chromatography, the hBD-2-inducing factor was identified as a 53-kDa monomeric protein with the amino-terminal sequence AQVINTNSLSLLTQNNLNK, which is identical to that of the flagella filament structural protein (FliC) of S. enteritidis. Consistent with this finding, the 53-kDa protein reacted with anti-FliC antibody, which prevented its induction of hBD-2 mRNA in Caco-2 cells. In agreement, the hBD-2-inducing activity in culture supernatant was completely neutralized by anti-FliC antibody. In gel retardation analyses, FliC increased binding of NF-kappaB (p65 homodimer) to hBD-2 gene promoter sequences. We conclude that S. enteritidis FliC induces hBD-2 expression in Caco-2 cells via NF-kappaB activation and thus plays an important role in up-regulation of the innate immune response. | lld:pubmed |
| pubmed-article:11387317 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:11387317 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11387317 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11387317 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11387317 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:11387317 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:AoyagiHH | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:MossJJ | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:WadeMM | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:OishiKK | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:MoriNN | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:NagatakeTT | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:HirayamaTT | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:TakahashiAA | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:AsakuraHH | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:HojoHH | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:NakaharaYY | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:OhsakiMM | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:HatakeyamaTT | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:NiidomeTT | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:KurazonoHH | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:Makino SS | lld:pubmed |
| pubmed-article:11387317 | pubmed:author | pubmed-author:Ogushi KK | lld:pubmed |
| pubmed-article:11387317 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11387317 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:11387317 | pubmed:volume | 276 | lld:pubmed |
| pubmed-article:11387317 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11387317 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11387317 | pubmed:pagination | 30521-6 | lld:pubmed |
| pubmed-article:11387317 | pubmed:dateRevised | 2010-9-17 | lld:pubmed |
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| pubmed-article:11387317 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11387317 | pubmed:articleTitle | Salmonella enteritidis FliC (flagella filament protein) induces human beta-defensin-2 mRNA production by Caco-2 cells. | lld:pubmed |
| pubmed-article:11387317 | pubmed:affiliation | Department of Bacteriology, Nagasaki University, Sakamoto, Nagasaki 852-8523, Japan. | lld:pubmed |
| pubmed-article:11387317 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11387317 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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