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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
32
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pubmed:dateCreated |
2001-8-6
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pubmed:abstractText |
Antimicrobial peptides are crucial for host defense at mucosal surfaces. Bacterial factors responsible for induction of human beta-defensin-2 (hBD-2) mRNA expression in Caco-2 human carcinoma cells were determined. Salmonella enteritidis, Salmonella typhimurium, Salmonella typhi, Salmonella dublin, and culture supernatants of these strains induced hBD-2 mRNA expression in Caco-2 human carcinoma cells. Using luciferase as a reporter gene for a approximately 2.1-kilobase pair hBD-2 promoter, the hBD-2-inducing factor in culture supernatant of S. enteritidis was isolated. The supernatant factor was heat-stable and proteinase-sensitive. After purification by anion exchange and gel filtration chromatography, the hBD-2-inducing factor was identified as a 53-kDa monomeric protein with the amino-terminal sequence AQVINTNSLSLLTQNNLNK, which is identical to that of the flagella filament structural protein (FliC) of S. enteritidis. Consistent with this finding, the 53-kDa protein reacted with anti-FliC antibody, which prevented its induction of hBD-2 mRNA in Caco-2 cells. In agreement, the hBD-2-inducing activity in culture supernatant was completely neutralized by anti-FliC antibody. In gel retardation analyses, FliC increased binding of NF-kappaB (p65 homodimer) to hBD-2 gene promoter sequences. We conclude that S. enteritidis FliC induces hBD-2 expression in Caco-2 cells via NF-kappaB activation and thus plays an important role in up-regulation of the innate immune response.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
pubmed-author:AoyagiHH,
pubmed-author:AsakuraHH,
pubmed-author:HatakeyamaTT,
pubmed-author:HirayamaTT,
pubmed-author:HojoHH,
pubmed-author:KurazonoHH,
pubmed-author:Makino SS,
pubmed-author:MoriNN,
pubmed-author:MossJJ,
pubmed-author:NagatakeTT,
pubmed-author:NakaharaYY,
pubmed-author:NiidomeTT,
pubmed-author:Ogushi KK,
pubmed-author:OhsakiMM,
pubmed-author:OishiKK,
pubmed-author:TakahashiAA,
pubmed-author:WadeMM
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
30521-6
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pubmed:dateRevised |
2010-9-17
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pubmed:meshHeading |
pubmed-meshheading:11387317-Amino Acid Sequence,
pubmed-meshheading:11387317-Blotting, Western,
pubmed-meshheading:11387317-Caco-2 Cells,
pubmed-meshheading:11387317-Cell Nucleus,
pubmed-meshheading:11387317-Chromatography, Gel,
pubmed-meshheading:11387317-Chromatography, Ion Exchange,
pubmed-meshheading:11387317-Dimerization,
pubmed-meshheading:11387317-Dose-Response Relationship, Drug,
pubmed-meshheading:11387317-Enzyme Activation,
pubmed-meshheading:11387317-Escherichia coli,
pubmed-meshheading:11387317-Flagellin,
pubmed-meshheading:11387317-Gene Deletion,
pubmed-meshheading:11387317-Humans,
pubmed-meshheading:11387317-Infection,
pubmed-meshheading:11387317-Luciferases,
pubmed-meshheading:11387317-Molecular Sequence Data,
pubmed-meshheading:11387317-NF-kappa B,
pubmed-meshheading:11387317-Promoter Regions, Genetic,
pubmed-meshheading:11387317-Protein Binding,
pubmed-meshheading:11387317-RNA, Messenger,
pubmed-meshheading:11387317-Recombinant Proteins,
pubmed-meshheading:11387317-Salmonella,
pubmed-meshheading:11387317-Salmonella enteritidis,
pubmed-meshheading:11387317-Transfection,
pubmed-meshheading:11387317-Up-Regulation,
pubmed-meshheading:11387317-beta-Defensins
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pubmed:year |
2001
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pubmed:articleTitle |
Salmonella enteritidis FliC (flagella filament protein) induces human beta-defensin-2 mRNA production by Caco-2 cells.
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pubmed:affiliation |
Department of Bacteriology, Nagasaki University, Sakamoto, Nagasaki 852-8523, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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