Linked Life Data

11386615

Source:http://linkedlifedata.com/resource/pubmed/id/11386615

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2001-6-1
pubmed:abstractText
Heme oxygenase (HO) plays a pivotal role for the maintenance of liver blood flow and hepatocellular integrity after hemorrhagic shock. We investigated the role of Kupffer cells and neutrophils as paracrine modulators of hepatocellular HO-1 gene expression in a rat model of hemorrhage and resuscitation. Male Sprague-Dawley rats (n = 6-10/group) were anesthetized (pentobarbital, 50 mg/kg intraperitonal) and subjected to hemorrhagic shock (mean arterial blood pressure: 35 mmHg for 60 min) or a sham protocol. Based on the time course of HO-1 gene expression, the effect of various antioxidants, Kupffer cell blockade [gadolinium chloride (GdCl3); 10 mg/kg; 24 h prior to hemorrhage or dichloromethylene diphosphonate (Cl2MDP); 1 mg/kg; 2 days prior to hemorrhage], or neutrophil depletion (vinblastine, 0.5 mg/kg, 5 days prior to hemorrhage) on induction of the HO-1 gene was assessed at 5 h of resuscitation, i.e., the time point of maximal induction. Kupffer cell blockade and antioxidants abolished HO-1 mRNA and protein induction after hemorrhage, while neutrophil depletion failed to affect hepatocellular HO-1 gene expression. In addition, Kupffer cell blockade aggravated hepatocellular injury. N-formyl-methionine-leucyl-phenylalanin (fMLP) induced a substantial influx of neutrophils into the liver but failed to induce hepatocellular HO-1 mRNA expression. These data suggest that Kupffer cells but not neutrophils induce an adaptive hepatocellular stress response after hemorrhage and resuscitation. Oxygen-free radicals released by Kupffer cells may serve as paracrine regulators of a hepatocellular stress gene which is necessary to maintain liver blood flow and integrity under stress conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
438-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11386615-Acetylcysteine, pubmed-meshheading:11386615-Animals, pubmed-meshheading:11386615-Antioxidants, pubmed-meshheading:11386615-Blood Pressure, pubmed-meshheading:11386615-Cells, Cultured, pubmed-meshheading:11386615-Chromans, pubmed-meshheading:11386615-Clodronic Acid, pubmed-meshheading:11386615-Deferoxamine, pubmed-meshheading:11386615-Disease Models, Animal, pubmed-meshheading:11386615-Gadolinium, pubmed-meshheading:11386615-Gene Expression Regulation, Enzymologic, pubmed-meshheading:11386615-Heme Oxygenase (Decyclizing), pubmed-meshheading:11386615-Heme Oxygenase-1, pubmed-meshheading:11386615-Hepatocytes, pubmed-meshheading:11386615-Kupffer Cells, pubmed-meshheading:11386615-Male, pubmed-meshheading:11386615-Neutrophils, pubmed-meshheading:11386615-Rats, pubmed-meshheading:11386615-Rats, Sprague-Dawley, pubmed-meshheading:11386615-Resuscitation, pubmed-meshheading:11386615-Shock, Hemorrhagic, pubmed-meshheading:11386615-Time Factors, pubmed-meshheading:11386615-Vinblastine
pubmed:year
2001
pubmed:articleTitle
Kupffer cells and neutrophils as paracrine regulators of the heme oxygenase-1 gene in hepatocytes after hemorrhagic shock.
pubmed:affiliation
Department of Anesthesiology and Critical Care Medicine, University of the Saarland, Homburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't