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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-5-31
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pubmed:abstractText |
The t(14;18) translocation is a useful marker to characterize follicular lymphoma and to monitor residual disease. The polymerase chain reaction (PCR) is a powerful technique to detect this translocation. Located on chromosome 18, within the Bcl-2 gene, breakpoints occur mainly in the 3; untranslated region, in the third exon of Bcl-2 (MBR region). In this study, the authors amplified MBR breakpoints by PCR and found an unexpectedly large fragment of 1 Kb that corresponds to a recently described new breakpoint in the Bcl-2 gene. With a new primer set, a further previously considered t(14;18)-unrelated tumor was in fact positive for this new breakpoint.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1052-9551
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-94
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11385316-Base Sequence,
pubmed-meshheading:11385316-Blotting, Southern,
pubmed-meshheading:11385316-Chromosome Breakage,
pubmed-meshheading:11385316-Chromosomes, Human, Pair 14,
pubmed-meshheading:11385316-Chromosomes, Human, Pair 18,
pubmed-meshheading:11385316-DNA, Neoplasm,
pubmed-meshheading:11385316-DNA Primers,
pubmed-meshheading:11385316-Electrophoresis, Agar Gel,
pubmed-meshheading:11385316-Genes, bcl-2,
pubmed-meshheading:11385316-Humans,
pubmed-meshheading:11385316-Immunoenzyme Techniques,
pubmed-meshheading:11385316-Lymph Nodes,
pubmed-meshheading:11385316-Lymphoma, Follicular,
pubmed-meshheading:11385316-Molecular Sequence Data,
pubmed-meshheading:11385316-Oligonucleotide Probes,
pubmed-meshheading:11385316-Polymerase Chain Reaction,
pubmed-meshheading:11385316-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:11385316-Translocation, Genetic
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pubmed:year |
2001
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pubmed:articleTitle |
A 1-kb Bcl-2-PCR fragment detection in a patient with follicular lymphoma and development of a new diagnostic PCR method.
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pubmed:affiliation |
Department of Molecular Oncology, Institut Bergonié, Bordeaux, France. ihostein@hotmail.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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