Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5 Suppl
pubmed:dateCreated
2001-5-30
pubmed:abstractText
Recent studies indicate an important role of endogenous oxidative stress in the onset and/or in the progression of mitochondrial encephalomyopathies. In particular, the increased production of radical species caused by altered mitochondrial functionality could affect both mitochondrial and nuclear DNA integrity. We performed the micronucleus assay coupled with fluorescence in situ hybridization (FISH) to detect chromosome damage in peripheral blood lymphocytes in a group of patients affected by different forms of mitochondrial encephalomyopathies. Moreover the comet assay has been carried out to detect primary and oxidative damage in the nuclear DNA. Our results show a significant presence of both DNA and chromosome damage in patients compared to a matched group of controls. A reduction in DNA alterations is also observed in patients after treatment with coenzyme-Q10.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1590-1874
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S963-5
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11382196-Adult, pubmed-meshheading:11382196-Aged, pubmed-meshheading:11382196-Chromosome Aberrations, pubmed-meshheading:11382196-Chromosome Disorders, pubmed-meshheading:11382196-Coenzymes, pubmed-meshheading:11382196-Comet Assay, pubmed-meshheading:11382196-DNA, pubmed-meshheading:11382196-DNA Damage, pubmed-meshheading:11382196-DNA Mutational Analysis, pubmed-meshheading:11382196-Female, pubmed-meshheading:11382196-Humans, pubmed-meshheading:11382196-Lymphocytes, pubmed-meshheading:11382196-Male, pubmed-meshheading:11382196-Micronucleus Tests, pubmed-meshheading:11382196-Middle Aged, pubmed-meshheading:11382196-Mitochondrial Encephalomyopathies, pubmed-meshheading:11382196-Oxidative Stress, pubmed-meshheading:11382196-Treatment Outcome, pubmed-meshheading:11382196-Ubiquinone
pubmed:year
2000
pubmed:articleTitle
Cytogenetic damage in peripheral lymphocytes of mitochondrial disease patients.
pubmed:affiliation
Department of Human and Environmental Sciences, University of Pisa, Via S. Giuseppe 22, Pisa, Italy.
pubmed:publicationType
Journal Article