Linked Life Data

11380423

Source:http://linkedlifedata.com/resource/pubmed/id/11380423

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-5-30
pubmed:abstractText
Resveratrol, a natural dietary polyphenol, has been postulated to be implicated in the cardioprotective effect of red wine and the low incidence of breast and prostate cancers among vegetarians and Orientals respectively. This compound inhibits ribonucleotide reductase as does hydroxyurea, the first therapeutic agent used in the treatment of sickle cell disease. Using the human erythroleukaemic K562 cell line as an in vitro model, we show here that 50 micromol/l of resveratrol induced a higher haemoglobin production (sevenfold) in K562 cells than 500 micromol/l of hydroxyurea (3.5-fold). This erythroid differentiation was linked to a dose- and time-dependent inhibition of cell proliferation associated with an equivalent increased expression of p21 mRNA, but with a higher increased level of p21 protein (sixfold) for cells treated with resveratrol than for those treated with hydroxyurea (1.5-fold). We also show that 50 micromol/l of resveratrol and 25 micromol/l of hydroxyurea induced variable but similar enhancements of fetal haemoglobin synthesis in cultured erythroid progenitors for the majority of the sickle cell patients studied. These inductions were linked to, but not correlated with, a variable decrease in erythroid burst-forming unit clone number. Taken together, these results show that resveratrol merits further investigations in sickle cell disease therapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0007-1048
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
500-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11380423-Anemia, Sickle Cell, pubmed-meshheading:11380423-Antioxidants, pubmed-meshheading:11380423-Blotting, Western, pubmed-meshheading:11380423-Cell Differentiation, pubmed-meshheading:11380423-Cell Division, pubmed-meshheading:11380423-Cell Line, pubmed-meshheading:11380423-Cells, Cultured, pubmed-meshheading:11380423-Dose-Response Relationship, Drug, pubmed-meshheading:11380423-Enzyme Inhibitors, pubmed-meshheading:11380423-Erythroid Precursor Cells, pubmed-meshheading:11380423-Fetal Hemoglobin, pubmed-meshheading:11380423-Gene Expression, pubmed-meshheading:11380423-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:11380423-Hemoglobins, pubmed-meshheading:11380423-Humans, pubmed-meshheading:11380423-Hydroxyurea, pubmed-meshheading:11380423-Leukemia, Erythroblastic, Acute, pubmed-meshheading:11380423-Models, Biological, pubmed-meshheading:11380423-Proto-Oncogene Proteins p21(ras), pubmed-meshheading:11380423-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:11380423-Ribonucleotide Reductases, pubmed-meshheading:11380423-Stem Cells, pubmed-meshheading:11380423-Stilbenes, pubmed-meshheading:11380423-Time Factors
pubmed:year
2001
pubmed:articleTitle
Resveratrol, a natural dietary phytoalexin, possesses similar properties to hydroxyurea towards erythroid differentiation.
pubmed:affiliation
INSERM U474, Maternité Port-Royal, ICGM Cochin, Paris, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't