Linked Life Data

11378413

Source:http://linkedlifedata.com/resource/pubmed/id/11378413

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-5-29
pubmed:abstractText
The present work analyzed the function of lipid transfer particle (LTP) in the process of exporting diacylglycerol from larval Manduca sexta midgut cells to lipophorin. When midgut sacs, which had been prelabeled in vivo with [(3)H]oleic acid, were incubated in vitro with a lipophorin-containing medium, a significant amount of radiolabeled diacylglycerol was transferred to lipophorin. Negligible amounts of diacylglycerol were released into lipophorin-free medium. In contrast, lipid-labeled lipophorin did not transfer diacylglycerol to the midgut sacs. The transfer of diacylglycerol from the midgut sac to lipophorin was blocked by preincubation of midgut sacs with antibody against LTP. Diacylglycerol transfer was restored to control values by the addition of purified LTP to midgut sacs that had been treated with antibody against LTP. Under these conditions the amount of diacylglycerol transferred was a function of the LTP concentration. These are the first results showing that LTP is required to export diacylglycerol from the midgut to lipophorin.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0965-1748
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
783-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Role of lipid transfer particle in delivery of diacylglycerol from midgut to lipophorin in larval Manduca sexta.
pubmed:affiliation
Department of Biochemistry and Molecular Biophysics and Center for Insect Science, Biological Sciences West, The University of Arizona, Tucson, AZ 85721-0088, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.