Linked Life Data

11375977

Source:http://linkedlifedata.com/resource/pubmed/id/11375977

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
30
pubmed:dateCreated
2001-7-23
pubmed:abstractText
The transcriptional enhancer (E(mu)3') of the IgH locus of the channel catfish, Ictalurus punctatus, shows strong B cell-specific activity and differs from the mammalian E(mu) enhancer in both location and structure. It occurs between the mu and delta genes and contains numerous transcription factor binding sites, predominantly octamer and muE5 motifs of consensus and variant sequences. It lacks the classical muA-muE3(CBF)-muB core array of binding motifs seen within mammalian IgH E(mu) enhancers. To determine the functionally important motifs, a series of mutant enhancers was created using sequence-targeted polymerase chain reaction. Whereas the mutation of consensus and variant octamer motifs (individually or in multiples) decreased enhancer function, mutation of a single consensus muE5 motif destroyed the function of this enhancer in mammalian plasmacytomas. Mutation of this consensus muE5 site, combined with mutations of certain octamer sites, destroyed function in catfish B cells. Experiments using artificial enhancers containing multimers of motifs or short regions of the native enhancer suggested that the minimal E(mu)3' enhancer (a) contains a consensus muE5 site and two octamer sites, (b) is B cell-specific, and (c) is active across species. The dependence of an Ig enhancer on sites that bind basic helix-loop-helix and Oct transcription factors has not previously been observed and confirms large differences in structure and function between fish and mammalian IgH enhancers.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27825-30
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11375977-Amino Acid Motifs, pubmed-meshheading:11375977-Animals, pubmed-meshheading:11375977-Binding Sites, pubmed-meshheading:11375977-Catfishes, pubmed-meshheading:11375977-Cell Line, pubmed-meshheading:11375977-DNA-Binding Proteins, pubmed-meshheading:11375977-Enhancer Elements, Genetic, pubmed-meshheading:11375977-Genes, Reporter, pubmed-meshheading:11375977-Helix-Loop-Helix Motifs, pubmed-meshheading:11375977-Host Cell Factor C1, pubmed-meshheading:11375977-Immunoglobulin D, pubmed-meshheading:11375977-Immunoglobulin M, pubmed-meshheading:11375977-Immunoglobulins, pubmed-meshheading:11375977-Mutagenesis, Site-Directed, pubmed-meshheading:11375977-Mutation, pubmed-meshheading:11375977-Octamer Transcription Factor-1, pubmed-meshheading:11375977-Physical Chromosome Mapping, pubmed-meshheading:11375977-Plasmids, pubmed-meshheading:11375977-Polymerase Chain Reaction, pubmed-meshheading:11375977-Transcription, Genetic, pubmed-meshheading:11375977-Transcription Factors, pubmed-meshheading:11375977-Transcriptional Activation, pubmed-meshheading:11375977-Transfection
pubmed:year
2001
pubmed:articleTitle
An IgH enhancer that drives transcription through basic helix-loop-helix and Oct transcription factor binding motifs. Functional analysis of the E(mu)3' enhancer of the catfish.
pubmed:affiliation
Department of Microbiology and Immunology, The Medical University of South Carolina, Charleston, South Carolina 29425, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.