Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-5-25
pubmed:abstractText
It was tested whether the inducible nitric oxide synthase (iNOS) pathway might be involved in lipopolysaccharides-(LPS)-induced up-regulation of L-arginine transport in rat alveolar macrophages (AM). AM were cultured in absence or presence of LPS. Nitrite accumulation was determined in culture media and cells were used to study [3H]-L-arginine uptake or to isolate RNA for RT - PCR. Culture in presence of LPS (1 microg ml(-1), 20 h) caused 11 fold increase of nitrite accumulation and 2.5 fold increase of [3H]-L-arginine uptake. The inducible NO synthase (iNOS) inhibitor 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine (AMT) present alone during culture had only marginal effects on [3H]-L-arginine uptake. However, AMT present during culture additionally to LPS, suppressed LPS-induced nitrite accumulation and LPS-stimulated [3H]-L-arginine uptake in the same concentration-dependent manner. AMT present only for the last 30 min of the culture period had similar effects on [3H]-L-arginine uptake. AMT present only during the uptake period also inhibited LPS-stimulated [3H]-L-arginine uptake, but with lower potency. The inhibitory effect of AMT could not be opposed by the NO releasing compound DETA NONOate. LPS caused an up-regulation of the mRNA for the cationic amino acid transporter CAT-2B, and this effect was not affected by AMT. AMT (100 microM) did not affect L-arginine transport studied by electrophysiological techniques in Xenopus laevis oocytes expressing either the human cationic amino acid transporter hCAT-1 or hCAT-2B. In conclusion, iNOS inhibition in rat AM abolished LPS-activated L-arginine uptake. This effect appears to be caused by reduced flow of L-arginine through the iNOS pathway.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-10333501, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-10617680, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-10882037, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-110886, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-11093766, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1373522, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1382188, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1599394, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1712021, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1715579, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1782986, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1852778, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-7533622, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-7536889, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-7545789, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-8075867, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-8532063, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9174363, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9178655, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9179379, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9308913, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9562037, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9714862, http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9879717
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/2-amino-5,6-dihydro-6-methyl-4H-1,3-..., http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems, Basic, http://linkedlifedata.com/resource/pubmed/chemical/Arginine, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/NOS2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II, http://linkedlifedata.com/resource/pubmed/chemical/Nitrites, http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Slc7a2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Thiazines
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
133
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-86
pubmed:dateRevised
2011-10-27
pubmed:meshHeading
pubmed-meshheading:11375254-Amino Acid Transport Systems, Basic, pubmed-meshheading:11375254-Animals, pubmed-meshheading:11375254-Arginine, pubmed-meshheading:11375254-Biological Transport, pubmed-meshheading:11375254-Carrier Proteins, pubmed-meshheading:11375254-Enzyme Inhibitors, pubmed-meshheading:11375254-Female, pubmed-meshheading:11375254-Humans, pubmed-meshheading:11375254-Lipopolysaccharides, pubmed-meshheading:11375254-Macrophages, Alveolar, pubmed-meshheading:11375254-Male, pubmed-meshheading:11375254-Membrane Potentials, pubmed-meshheading:11375254-Membrane Proteins, pubmed-meshheading:11375254-Nitric Oxide Synthase, pubmed-meshheading:11375254-Nitric Oxide Synthase Type II, pubmed-meshheading:11375254-Nitrites, pubmed-meshheading:11375254-Oocytes, pubmed-meshheading:11375254-RNA, Messenger, pubmed-meshheading:11375254-Rats, pubmed-meshheading:11375254-Rats, Sprague-Dawley, pubmed-meshheading:11375254-Signal Transduction, pubmed-meshheading:11375254-Thiazines, pubmed-meshheading:11375254-Xenopus laevis
pubmed:year
2001
pubmed:articleTitle
Inhibition of nitric oxide synthase abrogates lipopolysaccharides-induced up-regulation of L-arginine uptake in rat alveolar macrophages.
pubmed:affiliation
Institute of Pharmacology and Toxicology, University of Bonn, Reuterstr. 2b, D-53113 Bonn, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't