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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-5-25
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pubmed:abstractText |
It was tested whether the inducible nitric oxide synthase (iNOS) pathway might be involved in lipopolysaccharides-(LPS)-induced up-regulation of L-arginine transport in rat alveolar macrophages (AM). AM were cultured in absence or presence of LPS. Nitrite accumulation was determined in culture media and cells were used to study [3H]-L-arginine uptake or to isolate RNA for RT - PCR. Culture in presence of LPS (1 microg ml(-1), 20 h) caused 11 fold increase of nitrite accumulation and 2.5 fold increase of [3H]-L-arginine uptake. The inducible NO synthase (iNOS) inhibitor 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine (AMT) present alone during culture had only marginal effects on [3H]-L-arginine uptake. However, AMT present during culture additionally to LPS, suppressed LPS-induced nitrite accumulation and LPS-stimulated [3H]-L-arginine uptake in the same concentration-dependent manner. AMT present only for the last 30 min of the culture period had similar effects on [3H]-L-arginine uptake. AMT present only during the uptake period also inhibited LPS-stimulated [3H]-L-arginine uptake, but with lower potency. The inhibitory effect of AMT could not be opposed by the NO releasing compound DETA NONOate. LPS caused an up-regulation of the mRNA for the cationic amino acid transporter CAT-2B, and this effect was not affected by AMT. AMT (100 microM) did not affect L-arginine transport studied by electrophysiological techniques in Xenopus laevis oocytes expressing either the human cationic amino acid transporter hCAT-1 or hCAT-2B. In conclusion, iNOS inhibition in rat AM abolished LPS-activated L-arginine uptake. This effect appears to be caused by reduced flow of L-arginine through the iNOS pathway.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-10333501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-10617680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-10882037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-110886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-11093766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1373522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1382188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1599394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1712021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1715579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1782986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-1852778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-7533622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-7536889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-7545789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-8075867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-8532063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9174363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9178655,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9179379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9308913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9562037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9714862,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375254-9879717
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-amino-5,6-dihydro-6-methyl-4H-1,3-...,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems, Basic,
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NOS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrites,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Slc7a2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazines
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
133
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
379-86
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pubmed:dateRevised |
2011-10-27
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pubmed:meshHeading |
pubmed-meshheading:11375254-Amino Acid Transport Systems, Basic,
pubmed-meshheading:11375254-Animals,
pubmed-meshheading:11375254-Arginine,
pubmed-meshheading:11375254-Biological Transport,
pubmed-meshheading:11375254-Carrier Proteins,
pubmed-meshheading:11375254-Enzyme Inhibitors,
pubmed-meshheading:11375254-Female,
pubmed-meshheading:11375254-Humans,
pubmed-meshheading:11375254-Lipopolysaccharides,
pubmed-meshheading:11375254-Macrophages, Alveolar,
pubmed-meshheading:11375254-Male,
pubmed-meshheading:11375254-Membrane Potentials,
pubmed-meshheading:11375254-Membrane Proteins,
pubmed-meshheading:11375254-Nitric Oxide Synthase,
pubmed-meshheading:11375254-Nitric Oxide Synthase Type II,
pubmed-meshheading:11375254-Nitrites,
pubmed-meshheading:11375254-Oocytes,
pubmed-meshheading:11375254-RNA, Messenger,
pubmed-meshheading:11375254-Rats,
pubmed-meshheading:11375254-Rats, Sprague-Dawley,
pubmed-meshheading:11375254-Signal Transduction,
pubmed-meshheading:11375254-Thiazines,
pubmed-meshheading:11375254-Xenopus laevis
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pubmed:year |
2001
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pubmed:articleTitle |
Inhibition of nitric oxide synthase abrogates lipopolysaccharides-induced up-regulation of L-arginine uptake in rat alveolar macrophages.
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pubmed:affiliation |
Institute of Pharmacology and Toxicology, University of Bonn, Reuterstr. 2b, D-53113 Bonn, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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