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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-5-25
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pubmed:abstractText |
The toluene-degrading strain Rhodococcus opacus PWD4 was found to hydroxylate D-limonene exclusively in the 6-position, yielding enantiomerically pure (+) trans-carveol and traces of (+) carvone. This biotransformation was studied using cells cultivated in chemostat culture with toluene as a carbon and energy source. The maximal specific activity of (+) trans-carveol formation was 14.7 U (g of cells [dry weight])(-1), and the final yield was 94 to 97%. Toluene was found to be a strong competitive inhibitor of the D-limonene conversion. Glucose-grown cells did not form any trans-carveol from D-limonene. These results suggest that one of the enzymes involved in toluene degradation is responsible for this allylic monohydroxylation. Another toluene degrader (Rhodococcus globerulus PWD8) had a lower specific activity but was found to oxidize most of the formed trans-carveol to (+) carvone, allowing for the biocatalytic production of this flavor compound.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-10224006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-10361724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-10415126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-10473585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-10831450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-10864450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-1860851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-1919511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-3365392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-4227570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-4227571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-4314232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-8157604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11375201-8593060
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2829-32
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11375201-Biotransformation,
pubmed-meshheading:11375201-Culture Media,
pubmed-meshheading:11375201-Cyclohexenes,
pubmed-meshheading:11375201-Flavoring Agents,
pubmed-meshheading:11375201-Glucose,
pubmed-meshheading:11375201-Isomerism,
pubmed-meshheading:11375201-Monoterpenes,
pubmed-meshheading:11375201-Rhodococcus,
pubmed-meshheading:11375201-Species Specificity,
pubmed-meshheading:11375201-Terpenes,
pubmed-meshheading:11375201-Toluene
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pubmed:year |
2001
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pubmed:articleTitle |
Biotransformation of D-limonene to (+) trans-carveol by toluene-grown Rhodococcus opacus PWD4 cells.
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pubmed:affiliation |
Institute of Biotechnology, ETH Hönggerberg, HPT, CH 8093, Zürich, Switzerland. duetz@biotech.biol.ethz.ch
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pubmed:publicationType |
Journal Article
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