Linked Life Data

11374439

Source:http://linkedlifedata.com/resource/pubmed/id/11374439

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8-9
pubmed:dateCreated
2001-5-25
pubmed:abstractText
In adults, haemopoiesis is located in the bone marrow, where it is tightly regulated by cytokines and by a physical association of haemopoietic progenitors with the stroma. However, in pathological situations, haemopoiesis can be partly or fully dislodged to peripheral tissues. It is not clear which are the requirements for a given peripheral stroma to sustain haemopoiesis. Using the growth factor-dependent cell line FDC-P1, we have compared the myelopoietic capacities of a murine bone marrow-derived cell line S17, a liver inflammatory granuloma-derived stroma (GR) that sustains haemopoiesis, and normal skin fibroblasts (SF) that sustain neither survival nor proliferation of myeloid cells. All three stromas expressed mRNA for major haemopoietins with the exception of IL-3. Despite the incapacity of SF to sustain FDC-P1 cells, the biologically active GM-CSF could be recovered from all the studied stromas by treatment with high-salt buffers that release non-covalently bound molecules from stroma cells. Glycosaminoglycans purified from stromas had distinct effect on the GM-CSF-mediated proliferation of FDC-P1 cells: those purified from S17 and GR cells were stimulatory, whereas those obtained from SF cells were slightly stimulatory at low concentration, but inhibitory at the higher ones. We conclude that the quality of the stroma pericellular glycoconjugates is determinant for the ability of a given stroma to sustain myelopoiesis, even when biologically active haemopoietins are locally produced.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0248-4900
pubmed:author
pubmed:issnType
Print
pubmed:volume
92
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
605-14
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11374439-Animals, pubmed-meshheading:11374439-Cell Communication, pubmed-meshheading:11374439-Cell Division, pubmed-meshheading:11374439-Cells, Cultured, pubmed-meshheading:11374439-Coculture Techniques, pubmed-meshheading:11374439-Cytokines, pubmed-meshheading:11374439-Extracellular Space, pubmed-meshheading:11374439-Fibroblasts, pubmed-meshheading:11374439-Glycosaminoglycans, pubmed-meshheading:11374439-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:11374439-Growth Substances, pubmed-meshheading:11374439-Leukopoiesis, pubmed-meshheading:11374439-Mice, pubmed-meshheading:11374439-Myeloid Progenitor Cells, pubmed-meshheading:11374439-Proto-Oncogene Proteins c-kit, pubmed-meshheading:11374439-RNA, Messenger, pubmed-meshheading:11374439-Stromal Cells, pubmed-meshheading:11374439-Sulfur Radioisotopes
pubmed:year
2000
pubmed:articleTitle
The capacity of connective tissue stromas to sustain myelopoiesis depends both upon the growth factors and the local intercellular environment.
pubmed:affiliation
Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Cidade Universitária, Rio de Janeiro, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't