Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-5-25
pubmed:abstractText
Somatostatin hyperpolarized rat pancreatic alpha-cells and inhibited spontaneous electrical activity by activating a low-conductance K+ channel (0.9 pS with physiological ionic gradients). This channel was insensitive to tolbutamide (a blocker of ATP-sensitive K+ channels) and apamin (an inhibitor of small-conductance Ca(2+)-activated K+ channels). Channel activation was prevented by pre-treating the cells with pertussis toxin, indicating the involvement of G-proteins. A direct interaction between an inhibitory G-protein and the somatostatin-activated K+ channel is suggested by the finding that intracellular application of guanosine 5'-O-(3-thiotriphosphate) (GTP gamma-S) and the G beta gamma subunit of G-proteins resulted in a transient stimulation of the current. Activation of the K+ current by somatostatin was inhibited by intracellular dialysis with specific antibodies to Gi1/2 and was not seen in cells treated with antisense oligonucleotides against G-proteins of the subtype Gi2. We conclude that somatostatin suppresses alpha-cell electrical activity by a Gi2-protein-dependent mechanism, which culminates in the activation of a sulphonylurea- and apamin-insensitive low-conductance K+ channel.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, http://linkedlifedata.com/resource/pubmed/chemical/Apamin, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Protein alpha..., http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate), http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers, http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Somatostatin, http://linkedlifedata.com/resource/pubmed/chemical/Somatostatin, http://linkedlifedata.com/resource/pubmed/chemical/Tolbutamide, http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
442
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19-26
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11374064-Animals, pubmed-meshheading:11374064-Antibodies, pubmed-meshheading:11374064-Apamin, pubmed-meshheading:11374064-Calcium, pubmed-meshheading:11374064-Dialysis, pubmed-meshheading:11374064-Electric Conductivity, pubmed-meshheading:11374064-GTP-Binding Protein alpha Subunits, Gi-Go, pubmed-meshheading:11374064-Guanosine 5'-O-(3-Thiotriphosphate), pubmed-meshheading:11374064-Islets of Langerhans, pubmed-meshheading:11374064-Male, pubmed-meshheading:11374064-Pertussis Toxin, pubmed-meshheading:11374064-Potassium Channel Blockers, pubmed-meshheading:11374064-Potassium Channels, pubmed-meshheading:11374064-Rats, pubmed-meshheading:11374064-Rats, Inbred Lew, pubmed-meshheading:11374064-Receptors, Somatostatin, pubmed-meshheading:11374064-Somatostatin, pubmed-meshheading:11374064-Tolbutamide, pubmed-meshheading:11374064-Virulence Factors, Bordetella
pubmed:year
2001
pubmed:articleTitle
Gi2 proteins couple somatostatin receptors to low-conductance K+ channels in rat pancreatic alpha-cells.
pubmed:affiliation
Novo Nordisk A/S, Novo Alle, 2880 Bagsvaerd, Denmark. jesper_gromada@lilly.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't