Source:http://linkedlifedata.com/resource/pubmed/id/11373074
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-5-24
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| pubmed:abstractText |
Up-Converting Phosphor Technology (UPT) is based on lanthanide-containing, submicrometer-sized, ceramic particles that can absorb infrared light and emit visible light. Biological matrices do not up-convert; hence, there is no contribution to test background from sample autofluorescence. Up-converting phosphors do not photobleach and are inert to common assay interferants such as hemoglobin. A reader called UPlink has been developed to interrogate lateral flow test strips that utilize UPT labels. The reader contains a miniaturized, 1-W, infrared laser with peak emission at 980 nm. Preliminary assays that use up-converting phosphor labels, including tests for a drugs of abuse panel and Escherichia coli O157:H7, have been developed. In a "sandwich" assay format, 10(3) org/mL E. coli O157:H7 organisms were detectable in a negative control background of 10(9) other organisms per milliliter of culture medium. Coefficients of variation in concentrations tested from 0 to 10(7) org/mL were all < or =10%. In a competitive inhibition assay format, a multiplexed test simultaneously detected amphetamine, methamphetamine, phencyclidine, and opiates in saliva. For all assays, the percent displacement at 10 ng/mL was > or =40% demonstrating performance comparable with lab-based, commercially available EIAs. All assays were complete in 10 min. The development of rapid tests using UPT creates new applications for on-site testing with sensitivity not available using other label technologies.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Central Nervous System Stimulants,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Methamphetamine,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotics,
http://linkedlifedata.com/resource/pubmed/chemical/Phencyclidine
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0003-2697
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
293
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
22-30
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:11373074-Amphetamine-Related Disorders,
pubmed-meshheading:11373074-Antibodies, Bacterial,
pubmed-meshheading:11373074-Central Nervous System Stimulants,
pubmed-meshheading:11373074-Colorimetry,
pubmed-meshheading:11373074-Cross Reactions,
pubmed-meshheading:11373074-Enzyme Inhibitors,
pubmed-meshheading:11373074-Escherichia coli O157,
pubmed-meshheading:11373074-Humans,
pubmed-meshheading:11373074-Immunoenzyme Techniques,
pubmed-meshheading:11373074-Liposomes,
pubmed-meshheading:11373074-Luminescent Measurements,
pubmed-meshheading:11373074-Methamphetamine,
pubmed-meshheading:11373074-Microscopy, Electron,
pubmed-meshheading:11373074-Microscopy, Fluorescence,
pubmed-meshheading:11373074-Narcotic Antagonists,
pubmed-meshheading:11373074-Narcotics,
pubmed-meshheading:11373074-Phencyclidine,
pubmed-meshheading:11373074-Sensitivity and Specificity
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| pubmed:year |
2001
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| pubmed:articleTitle |
Detection of analytes by immunoassay using up-converting phosphor technology.
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| pubmed:affiliation |
OraSure Technologies, Inc., 150 Webster Street, Bethlehem, Pennsylvania 18015-1389, USA. sniedbala@orasure.com
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| pubmed:publicationType |
Journal Article
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