Linked Life Data

11369639

Source:http://linkedlifedata.com/resource/pubmed/id/11369639

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2001-5-22
pubmed:abstractText
Although the mouse spleen dendritic cell (DC) is perhaps the most intensively studied DC type, little has been published concerning its human equivalent. In this report, rare event flow cytometry and in situ immunofluorescence were used to study the surface phenotype and distribution of HLA-DR(+) CD3(-)14(-)16(-)19(-) human spleen DC. Spleens from organ donors with different clinical histories were used. Most (81% +/- 9%; n = 14) spleen DCs expressed high levels of the integrin CD11c. CD11c(+) DCs were distributed in 3 distinct regions-the peri-arteriolar T-cell zones, the B-cell zones, and the marginal zone, where they formed a ring of cells surrounding the white pulp, just inside a ring of CD14(+) red pulp macrophages, apparently more regularly organized than the previously described marginating DC population in the mouse spleen. The T-cell zones contained CD86(+) DCs, among which a subpopulation expressed CD83. These mature/activated CD86(+) DCs represented a minority (12% +/- 8%) of total spleen DCs in most organ donors: most spleen DCs are immature. In 3 of 18 (17%) donors, however, most (54%-81%) of spleen DCs were CD86(+), suggesting that in vivo DC activation had occurred. In one donor, a radical shift in DC distribution from the marginal zone to the T-cell zones was also observed. This activation of spleen DCs in vivo was reminiscent of the effects of experimental microbial product injection in mice, and it seemed to correlate with bacterial infection or multiple trauma. (Blood. 2001;97:3470-3477)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3470-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11369639-Adult, pubmed-meshheading:11369639-Antigens, CD, pubmed-meshheading:11369639-Antigens, CD11, pubmed-meshheading:11369639-Antigens, CD14, pubmed-meshheading:11369639-Antigens, CD3, pubmed-meshheading:11369639-Antigens, CD86, pubmed-meshheading:11369639-Dendritic Cells, pubmed-meshheading:11369639-Female, pubmed-meshheading:11369639-Flow Cytometry, pubmed-meshheading:11369639-Fluorescent Antibody Technique, pubmed-meshheading:11369639-HLA-DR Antigens, pubmed-meshheading:11369639-Humans, pubmed-meshheading:11369639-Immunoglobulins, pubmed-meshheading:11369639-Immunophenotyping, pubmed-meshheading:11369639-Interleukin-12, pubmed-meshheading:11369639-Male, pubmed-meshheading:11369639-Membrane Glycoproteins, pubmed-meshheading:11369639-Middle Aged, pubmed-meshheading:11369639-Phenotype, pubmed-meshheading:11369639-Spleen, pubmed-meshheading:11369639-Tissue Donors
pubmed:year
2001
pubmed:articleTitle
Investigation of human spleen dendritic cell phenotype and distribution reveals evidence of in vivo activation in a subset of organ donors.
pubmed:affiliation
Laboratoire d'Immunologie Cellulaire et Tissulaire URA CNRS 625, Service d'Urologie, Hôpital de La Pitié-Salpêtrière, Paris, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't