Source:http://linkedlifedata.com/resource/pubmed/id/11369584
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2001-5-22
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| pubmed:abstractText |
The myc family of transcriptional regulators carries out critical roles in the control of cellular proliferation, differentiation, apoptosis, and tumorigenesis. The B-myc gene is a recently identified myc family member that has not been well characterized. Previously, we have shown that B-Myc inhibits the ability of c-Myc to transform cells and can inhibit cellular proliferation. Because B-myc is primarily expressed in hormonally regulated tissues with predominant expression in the epididymis, we examined in greater detail B-myc expression in the epididymis to ultimately understand potential roles B-myc may play in this and other hormonally regulated tissues. Herein we demonstrate that, in contrast to c-myc, B-myc mRNA and protein expression are highly regionalized with expression predominantly in the proximal caput epididymal region. Furthermore, in situ and immunohistochemical analyses show that within the epididymis B-myc mRNA and protein are specifically expressed by the epithelial cells and that B-Myc protein is localized to both the nuclear and cytosolic compartments. Castration and hormone replacement studies further show that expression of the B-myc mRNA is highly dependent on the presence of androgens and testicular factors. Finally, mRNA turnover studies demonstrate that the B-myc mRNA is relatively unstable with a half-life of 3.5 h. Taken together, the highly restricted and regulated expression of the B-myc gene suggests it may play important regulatory roles in the epididymis and perhaps other hormonally regulated tissues.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androgens,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0006-3363
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
64
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1600-7
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11369584-Androgens,
pubmed-meshheading:11369584-Animals,
pubmed-meshheading:11369584-Blotting, Northern,
pubmed-meshheading:11369584-Cell Line,
pubmed-meshheading:11369584-Dactinomycin,
pubmed-meshheading:11369584-Drug Stability,
pubmed-meshheading:11369584-Epididymis,
pubmed-meshheading:11369584-Female,
pubmed-meshheading:11369584-Gene Expression,
pubmed-meshheading:11369584-Immunohistochemistry,
pubmed-meshheading:11369584-In Situ Hybridization,
pubmed-meshheading:11369584-Male,
pubmed-meshheading:11369584-Mice,
pubmed-meshheading:11369584-Mice, Inbred ICR,
pubmed-meshheading:11369584-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:11369584-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:11369584-RNA, Messenger,
pubmed-meshheading:11369584-Testis
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| pubmed:year |
2001
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| pubmed:articleTitle |
B-Myc, a proximal caput epididymal protein, is dependent on androgens and testicular factors for expression.
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| pubmed:affiliation |
Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA. gail.cornwall@ttmc.ttuhsc.edu
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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