Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2001-5-22
pubmed:abstractText
mRNA differential display-PCR analysis was used to perform a systematic screening of Somatostatin (SS)-regulated genes in the human prostatic carcinoma cell line LNCaP (Lymph Node Carcinoma of the Prostate). A 170 bp fragment was shown to be up-regulated by SS. Sequence analysis of this fragment revealed its homology with the human Topoisomerase II Alpha gene. Up-regulation of Topoisomerase II Alpha was confirmed by Northern blot hybridisation and was induced by the same dose of SS (1 nM) earlier demonstrated to inhibit LNCaP cell growth. Furthermore, SS possible effects on timing, as well as concentration of Topoisomerase II Alpha along the different phases of the cell cycle were investigated. To this purpose changes in the enzyme protein concentration in response to SS were assessed in synchronised LNCaP cells. The hormone was shown to exert a perturbing effect on both parameters considered, possibly related to its inhibitory action on LNCaP cell replication.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0303-7207
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
176
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
103-10
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:11369449-Antigens, Neoplasm, pubmed-meshheading:11369449-Base Sequence, pubmed-meshheading:11369449-Blotting, Northern, pubmed-meshheading:11369449-Blotting, Western, pubmed-meshheading:11369449-Cell Cycle, pubmed-meshheading:11369449-Cell Division, pubmed-meshheading:11369449-Cloning, Molecular, pubmed-meshheading:11369449-DNA Topoisomerases, Type II, pubmed-meshheading:11369449-DNA-Binding Proteins, pubmed-meshheading:11369449-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11369449-Gene Expression Regulation, Neoplastic, pubmed-meshheading:11369449-Humans, pubmed-meshheading:11369449-Isoenzymes, pubmed-meshheading:11369449-Male, pubmed-meshheading:11369449-Molecular Sequence Data, pubmed-meshheading:11369449-Polymerase Chain Reaction, pubmed-meshheading:11369449-Prostatic Neoplasms, pubmed-meshheading:11369449-RNA, Messenger, pubmed-meshheading:11369449-Sequence Homology, Amino Acid, pubmed-meshheading:11369449-Somatostatin, pubmed-meshheading:11369449-Tumor Cells, Cultured, pubmed-meshheading:11369449-Up-Regulation
pubmed:year
2001
pubmed:articleTitle
Somatostatin up-regulates topoisomerase II alpha expression and affects LNCaP cell cycle.
pubmed:affiliation
Centre for Endocrinological Oncology, Department of Endocrinology, University of Milan, Milan, Italy. tiziana.brevini@unimi.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't