Linked Life Data

11368313

Source:http://linkedlifedata.com/resource/pubmed/id/11368313

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-5-22
pubmed:abstractText
The limited ability of damaged muscle to regenerate after gross injuries is a major clinical problem. To date, there is no effective therapeutic treatment for muscle injuries. In the present study, we have examined the ability of crude and fractionated human skeletal muscle extracts to promote myogenic cell proliferation and differentiation. It was found that the crude muscle extract could significantly stimulate BrdU incorporation in C2C12 myogenic cell line. In addition, the extract also promoted myogenic cell alignment and fusion. Using electrophoresis techniques, in conjunction with in vitro refolding technique, a protein with molecular weight of approximately 40 kDa was identified that could produce the same effects as the crude muscle exdtract. We also tested the ability of semipurified (30-50 kDa) muscle extract to promote muscle repair in adult rats. Surgical intervention was used to induce muscle damage in the tibialis anterior. The semipurified muscle extract (fraction H) was injected subcutaneously over the tibialis anterior for a period of 5 days. It was found that the damaged muscle fibers were replaced by newly regenerated muscle fibers. These newly regenerated fibers originated from the fusion of differentiated satellite cells as revealed by BrdU-labeling analysis. In contrast, the injury site of muscles treated with BSA control protein contained mainly fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0003-9861
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
384
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
263-8
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Identification and purification of an intrinsic human muscle myogenic factor that enhances muscle repair and regeneration.
pubmed:affiliation
Department of Orthopaedics and Traumatology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT. kaimingchan@cuhk.edu.hk
pubmed:publicationType
Journal Article