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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1979-11-21
|
| pubmed:abstractText |
Three periods (the lag period and periods of intensive and decelerated spheroplast formation) can be detected in the action of lysozyme on the cells of Anacystis nidulans; this seems to be due to peculiarities in the cell wall structure of the cyanobacterium and heterogeneity of the culture. EDTA at concentrations of 0.175--0.7 mM has effect on the duration of the lag period but increases the rate of spheroplast formation during the subsequent periods. However, the action of EDTA in complex with lysozyme for 3 hours causes lysis of some of the spheroplasts being formed. The concentration of mannitol below 0.4 M decreases the yield of spheroplasts. The level of intact spheroplasts prepared from A. nidulans suggests that at least part of them is capable of reversing into whole cells.
|
| pubmed:language |
rus
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0026-3656
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
48
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
693-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:articleTitle |
[Effect of lysozyme, ethylenediaminetetraacetate, magnesium and mannittol on spheroplast formation in Anacystis nidulans].
|
| pubmed:publicationType |
Journal Article,
English Abstract
|