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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2001-5-21
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pubmed:abstractText |
Mammalian cells require a cyclin D-dependent kinase for the cell cycle start, yet many mesenchymal cells express three seemingly redundant D cyclins and similarly, seemingly redundant Cdk4 and Cdk6 as their kinase partners. We have found that the Cdk6-cyclin D3 complex is unique among the D cyclin and kinase combinations in the ability to promote the cell cycle start. In an anchorage-minus G(1)-arrested rat fibroblast, only Cdk6-D3 retains kinase activity due mainly to its ability to evade inhibition by p27(KIP1) and p21(CIP1) with a resemblance to viral cyclin-bound Cdk6. Rodent fibroblasts engineered to overexpress both Cdk6 and cyclin D3 highly resist serum starvation- or cell-cell contact-imposed G(1)-arrest. In BALB/c 3T3 cells, D3 is constitutively expressed, but Cdk6 is markedly induced with concomitant activation upon stimulation with a growth-promoting factor. These results suggest a role for the Cdk6-D3 complex in regulating cell's proliferation ability in response to external stimuli.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Ccnd3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ccnd3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk6 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1b protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1b protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D3,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 6,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
20
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2000-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11360184-3T3 Cells,
pubmed-meshheading:11360184-Animals,
pubmed-meshheading:11360184-Cell Adhesion,
pubmed-meshheading:11360184-Cell Cycle Proteins,
pubmed-meshheading:11360184-Cell Division,
pubmed-meshheading:11360184-Contact Inhibition,
pubmed-meshheading:11360184-Cyclin D3,
pubmed-meshheading:11360184-Cyclin-Dependent Kinase 6,
pubmed-meshheading:11360184-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:11360184-Cyclin-Dependent Kinase Inhibitor p27,
pubmed-meshheading:11360184-Cyclin-Dependent Kinases,
pubmed-meshheading:11360184-Cyclins,
pubmed-meshheading:11360184-G1 Phase,
pubmed-meshheading:11360184-Mice,
pubmed-meshheading:11360184-Mice, Inbred BALB C,
pubmed-meshheading:11360184-Mice, Inbred C3H,
pubmed-meshheading:11360184-Microtubule-Associated Proteins,
pubmed-meshheading:11360184-Platelet-Derived Growth Factor,
pubmed-meshheading:11360184-Precipitin Tests,
pubmed-meshheading:11360184-Protein-Serine-Threonine Kinases,
pubmed-meshheading:11360184-Rats,
pubmed-meshheading:11360184-Tetradecanoylphorbol Acetate,
pubmed-meshheading:11360184-Tumor Suppressor Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Cdk6-cyclin D3 complex evades inhibition by inhibitor proteins and uniquely controls cell's proliferation competence.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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