Source:http://linkedlifedata.com/resource/pubmed/id/11359806
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
2001-5-21
|
| pubmed:abstractText |
The culture supernatants of LK1 cells, murine erythroleukemia cells, showed B cell-stimulating activity. Purification and NH(2)-terminal sequence analysis revealed that one of the candidates was murine IgE-dependent histamine-releasing factor (IgE-HRF), which is known to induce histamine from basophils. Recombinant IgE-HRF (rHRF) obtained from Escherichia coli- or 293-transformed embryonal kidney cells was tested for B cell-stimulating activity. Both rHRFs stimulated B cell proliferation in a dose-dependent manner. However, boiling or anti-HRF Ab abolished the B cell stimulatory effects of rHRF. Recombinant HRF showed strong synergistic effects with IL-2, IL-4, and IL-5 for B cell activation, with maximal activity in the presence of anti-CD40 AB: Recombinant HRF increased MHC class II expression of B cells. It also increased Ig production from B cells. Treatment with polymyxin B, a neutralizing peptide antibiotic of LPS, did not reduce the activity of rHRF. In addition, FACS analysis using PE-conjugated rHRF showed that HRF bound to B cells. Recombinant HRF up-regulated the expression of IL-1 and IL-6 in B cells. In vivo administration of rHRF or the cDNA for rHRF increased total and Ag-specific Ig synthesis. Taken together, these results indicate that HRF stimulates B cell activation and function.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/translationally controlled tumor...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
166
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6545-54
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11359806-Animals,
pubmed-meshheading:11359806-B-Lymphocytes,
pubmed-meshheading:11359806-Cell Division,
pubmed-meshheading:11359806-Cell-Free System,
pubmed-meshheading:11359806-Cytokines,
pubmed-meshheading:11359806-Female,
pubmed-meshheading:11359806-Gene Expression Regulation,
pubmed-meshheading:11359806-Growth Substances,
pubmed-meshheading:11359806-Histamine,
pubmed-meshheading:11359806-Humans,
pubmed-meshheading:11359806-Immunoglobulin E,
pubmed-meshheading:11359806-Immunoglobulins,
pubmed-meshheading:11359806-Injections, Intramuscular,
pubmed-meshheading:11359806-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:11359806-Lymphocyte Activation,
pubmed-meshheading:11359806-Lymphokines,
pubmed-meshheading:11359806-Mice,
pubmed-meshheading:11359806-Mice, Inbred BALB C,
pubmed-meshheading:11359806-Protein Binding,
pubmed-meshheading:11359806-Tumor Cells, Cultured,
pubmed-meshheading:11359806-Tumor Markers, Biological
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Molecular identification of IgE-dependent histamine-releasing factor as a B cell growth factor.
|
| pubmed:affiliation |
Laboratory of Immunology, Korea Research Institute of Bioscience and Biotechnology, Eoun-Dong 52, Yusong, Taejon 305-333, Republic of Korea.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|