Linked Life Data

11358534

Source:http://linkedlifedata.com/resource/pubmed/id/11358534

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-5-18
pubmed:abstractText
T-cell cytokine profiles, anti-Porphyromonas gingivalis antibodies and Western blot analysis of antibody responses were examined in BALB/c, CBA/CaH, C57BL6 and DBA/2J mice immunized intraperitoneally with different doses of P. gingivalis outer membrane antigens. Splenic CD4 and CD8 cells were examined for intracytoplasmic interleukin (IL)-4, interferon (IFN)-gamma and IL-10 by FACS analysis and levels of anti-P. gingivalis antibodies in the serum samples determined by enzyme-linked immunosorbent assay. Western blot analysis was performed on the sera from mice immunized with 100 microg of P. gingivalis antigens. The four strains of mice demonstrated varying degrees of T-cell immunity, although the T-cell cytokine profiles exhibited by each strain were not affected by different immunizing doses. While BALB/c and DBA/2J mice exhibited responses that peaked at immunizing doses of 100-200 microg of P. gingivalis antigens, CBA/CaH and C57BL6 demonstrated weak T-cell responsiveness compared with control mice. Like the T-cell responses, serum antibody levels were not dose dependent. DBA/2J exhibited the lowest levels of anti-P. gingivalis antibodies followed by BALB/c with CBA/CaH and C57BL6 mice demonstrating the highest levels. Western blot analysis showed that there were differences in reactivity between the strains to a group of 13 antigens ranging in molecular weight from 15 to 43 kDa. Antibody responses to a number of these bands in BALB/c mice were of low density, whereas CBA/CaH and C57BL6 mice demonstrated high-density bands and DBA/2J mice showed medium to high responses. In conclusion, different immunizing doses of P. gingivalis outer membrane antigens had little effect on the T-cell cytokine responses and serum anti-P. gingivalis antibody levels. Western blot analysis, however, indicated that the four strains of mice exhibited different reactivity to some lower-molecular-weight antigens. Future studies are required to determine the significance of these differences, which may affect the outcome of P. gingivalis infection.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0902-0055
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-35
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11358534-Analysis of Variance, pubmed-meshheading:11358534-Animals, pubmed-meshheading:11358534-Antibodies, Bacterial, pubmed-meshheading:11358534-Antigen-Antibody Reactions, pubmed-meshheading:11358534-Antigens, Bacterial, pubmed-meshheading:11358534-Bacterial Outer Membrane Proteins, pubmed-meshheading:11358534-Blotting, Western, pubmed-meshheading:11358534-CD4-Positive T-Lymphocytes, pubmed-meshheading:11358534-CD8-Positive T-Lymphocytes, pubmed-meshheading:11358534-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:11358534-Flow Cytometry, pubmed-meshheading:11358534-Genetic Variation, pubmed-meshheading:11358534-Immunization, pubmed-meshheading:11358534-Immunoglobulin G, pubmed-meshheading:11358534-Immunoglobulin M, pubmed-meshheading:11358534-Interferon-gamma, pubmed-meshheading:11358534-Interleukin-10, pubmed-meshheading:11358534-Interleukin-4, pubmed-meshheading:11358534-Mice, pubmed-meshheading:11358534-Mice, Inbred BALB C, pubmed-meshheading:11358534-Mice, Inbred C57BL, pubmed-meshheading:11358534-Mice, Inbred CBA, pubmed-meshheading:11358534-Mice, Inbred DBA, pubmed-meshheading:11358534-Mice, Inbred Strains, pubmed-meshheading:11358534-Molecular Weight, pubmed-meshheading:11358534-Porphyromonas gingivalis, pubmed-meshheading:11358534-Spleen
pubmed:year
2001
pubmed:articleTitle
Genetic variation in the recognition of Porphyromonas gingivalis antigens in mice.
pubmed:affiliation
Immunopathology Laboratory, Oral Biology and Pathology, School of Dentistry, University of Queensland, Brisbane, Australia.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't