Linked Life Data

11357319

Source:http://linkedlifedata.com/resource/pubmed/id/11357319

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-5-18
pubmed:abstractText
Agar media for isolation of enterohaemorrhagic Escherichia coli (EHEC) have been developed primarily for E. coli O157, because this bacterium has most frequently caused EHEC infection. However, there have been few studies for isolation of other serotypes of EHEC, and media appropriate for isolation of such organisms, especially from food samples, are not yet available. Among such serotypes, E. coli O26 has often been isolated from clinical specimens from patients and animals, but not from food samples in outbreaks, because of lack of an appropriate method for isolation. In this study, we tried to develop a new chromogenic agar medium for selective isolation of E. coli O26 using the characteristics of E. coli O26. Fifteen strains of E. coli O26, 11 strains of E. coli O157 and 36 strains of other sero-types E. coli were tested for fermentation of rhamnose, cellobiose, dulcitol, salicin, raffinose, sorbitol, sucrose, lactose, mannitol, arabinose, maltose, xylose and glucose. Rhamnose was fermented by all E. coli strains except for E. coli O26. The other substrates were not effective for differentiating E. coli O26 from the other strains of E. coli. Thus the medium containing rhamnose and 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, which is a substrate of beta-galactosidase specific to coliforms, produced a color of E. coli O26 colonies different from colors of the other bacteria. Furthermore, cefixime and sodium tellulite were added to the composition of the medium for gaining higher selectivity.
pubmed:language
jpn
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0387-5911
pubmed:author
pubmed:issnType
Print
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
291-9
pubmed:dateRevised
2008-12-16
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[Development of chromogenic agar medium for isolation of enterohaemorrhagic Escherichia coli O26].
pubmed:affiliation
University of Tokyo.
pubmed:publicationType
Journal Article, English Abstract