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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-5-17
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pubmed:abstractText |
The cardiac M2 muscarinic receptor/G protein/K+ channel system was studied in neonatal rat atrial cells cultured with and without 10 microM carbachol (CCh) for 24 h. Channel activity in CCh-pretreated cells was substantially reduced as a result of long-term desensitization regardless of whether the channel was activated by ACh in cell-attached patches or GTP in inside-out patches. Channel activity in CCh-pretreated cells was also low when the receptor was bypassed and the G protein and channel were directly activated by [gamma-S]GTP or both the receptor and G protein were bypassed and the channel was directly activated by trypsin. Finally, in CCh-pretreated cells, the whole cell K+ current was low when the channel was activated via the independent adenosine receptor. This suggests that the channel is involved in long-term desensitization. However, in CCh-pretreated cells, although the receptor was internalized, there was no internalization of the channel. We suggest that the function of the muscarinic K+ channel declines in long-term desensitization of the cardiac M2 muscarinic receptor/G protein/K+ channel system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/G Protein-Coupled...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine 5'-O-(3-Thiotriphosphate),
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Muscarinic M2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
280
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H2554-62
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11356610-Acetylcholine,
pubmed-meshheading:11356610-Animals,
pubmed-meshheading:11356610-Animals, Newborn,
pubmed-meshheading:11356610-CHO Cells,
pubmed-meshheading:11356610-Carbachol,
pubmed-meshheading:11356610-Cells, Cultured,
pubmed-meshheading:11356610-Cricetinae,
pubmed-meshheading:11356610-G Protein-Coupled Inwardly-Rectifying Potassium Channels,
pubmed-meshheading:11356610-GTP-Binding Proteins,
pubmed-meshheading:11356610-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:11356610-Guanosine Triphosphate,
pubmed-meshheading:11356610-Heart Atria,
pubmed-meshheading:11356610-Membrane Potentials,
pubmed-meshheading:11356610-Patch-Clamp Techniques,
pubmed-meshheading:11356610-Potassium,
pubmed-meshheading:11356610-Potassium Channels,
pubmed-meshheading:11356610-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:11356610-Rats,
pubmed-meshheading:11356610-Receptor, Muscarinic M2,
pubmed-meshheading:11356610-Receptors, Muscarinic,
pubmed-meshheading:11356610-Time Factors,
pubmed-meshheading:11356610-Transfection,
pubmed-meshheading:11356610-Trypsin
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pubmed:year |
2001
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pubmed:articleTitle |
Evidence of involvement of GIRK1/GIRK4 in long-term desensitization of cardiac muscarinic K+ channels.
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pubmed:affiliation |
School of Biomedical Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom.
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pubmed:publicationType |
Journal Article
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