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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2001-5-17
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pubmed:databankReference |
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pubmed:abstractText |
We sequenced a gene encoding AP endonuclease DdAPN in Dictyostelium discoideum. The sequence predicts a protein of 542 amino acids, showing high homology to Escherichia coli Endonuclease IV (Endo IV). There is 45% identity to Endo IV using the C-terminal 282 amino acids of the Dictyostelium protein. The DdAPN conserves nine residues for the metal-binding identified in Endo IV. The truncated DdAPN protein containing these sites partially complemented E. coli RPC501 (xth(-), nfo(-)).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-5107
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
486
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11356336-Amino Acid Sequence,
pubmed-meshheading:11356336-Animals,
pubmed-meshheading:11356336-Base Sequence,
pubmed-meshheading:11356336-Carbon-Oxygen Lyases,
pubmed-meshheading:11356336-Conserved Sequence,
pubmed-meshheading:11356336-DNA Damage,
pubmed-meshheading:11356336-DNA-(Apurinic or Apyrimidinic Site) Lyase,
pubmed-meshheading:11356336-Deoxyribonuclease IV (Phage T4-Induced),
pubmed-meshheading:11356336-Dictyostelium,
pubmed-meshheading:11356336-Dose-Response Relationship, Drug,
pubmed-meshheading:11356336-Escherichia coli,
pubmed-meshheading:11356336-Escherichia coli Proteins,
pubmed-meshheading:11356336-Hydrogen Peroxide,
pubmed-meshheading:11356336-Mitomycin,
pubmed-meshheading:11356336-Molecular Sequence Data,
pubmed-meshheading:11356336-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:11356336-Plasmids,
pubmed-meshheading:11356336-Sequence Homology, Amino Acid
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pubmed:year |
2001
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pubmed:articleTitle |
Endonuclease IV homolog from Dictyostelium discoideum: sequencing and functional expression in AP endonuclease-deficient Escherichia coli.
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pubmed:affiliation |
Division of Bioengineering, Faculty of Engineering, Toyama University, 930-8555, Toyama, Japan.
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pubmed:publicationType |
Journal Article
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