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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2001-5-17
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pubmed:abstractText |
The endogenous dopamine-derived neurotoxin salsolinol was found to decrease survival in the dopaminergic neuronal cell line RCSN-3, derived from adult rat substantia nigra in a concentration-dependent manner (208 microM salsolinol induced a 50% survival decrease). Incubation of RCSN-3 cells with 100 micro;M dicoumarol and salsolinol significantly decreased cell survival by 2.5-fold (P < 0.001), contrasting with a negligible effect on RCHT cells, which exhibited nearly a 5-fold lower nomifensine-insensitive dopamine uptake. The levels of catalase and glutathione peroxidase mRNA were decreased when RCSN-3 cells were treated with 100 microM salsolinol alone or in the presence of 100 microM dicoumarol. In vitro oxidation of salsolinol to o-quinone catalyzed by lactoperoxidase gave the quinone methide and 1,2-dihydro-1-methyl-6,7-isoquinoline diol as final products of salsolinol oxidation as determined by NMR analysis. Evidence of the formation of salsolinol o-semiquinone radical has been provided by ESR studies during one-electron oxidation of salsolinol catalyzed by lactoperoxidase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Catalase,
http://linkedlifedata.com/resource/pubmed/chemical/Dicumarol,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Indolequinones,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Isoquinolines,
http://linkedlifedata.com/resource/pubmed/chemical/Quinones,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxide Dismutase,
http://linkedlifedata.com/resource/pubmed/chemical/quinone methide,
http://linkedlifedata.com/resource/pubmed/chemical/salsolinol
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
283
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1069-76
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11355881-Animals,
pubmed-meshheading:11355881-Biological Transport,
pubmed-meshheading:11355881-Catalase,
pubmed-meshheading:11355881-Cell Line,
pubmed-meshheading:11355881-Cell Survival,
pubmed-meshheading:11355881-Dicumarol,
pubmed-meshheading:11355881-Dopamine,
pubmed-meshheading:11355881-Electron Spin Resonance Spectroscopy,
pubmed-meshheading:11355881-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11355881-Glutathione Peroxidase,
pubmed-meshheading:11355881-Indolequinones,
pubmed-meshheading:11355881-Indoles,
pubmed-meshheading:11355881-Isoquinolines,
pubmed-meshheading:11355881-Neurons,
pubmed-meshheading:11355881-Quinones,
pubmed-meshheading:11355881-RNA, Messenger,
pubmed-meshheading:11355881-Rats,
pubmed-meshheading:11355881-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11355881-Substantia Nigra,
pubmed-meshheading:11355881-Superoxide Dismutase,
pubmed-meshheading:11355881-Transcription, Genetic
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pubmed:year |
2001
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pubmed:articleTitle |
Possible role of salsolinol quinone methide in the decrease of RCSN-3 cell survival.
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pubmed:affiliation |
Programme of Molecular and Clinical Pharmacology, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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