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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2001-5-17
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pubmed:abstractText |
Total collagen assays are often laborious and use large quantities of consumables. We have developed a new method of assaying total 3H-proline-labeled collagen from cultured cells. Cells and media are harvested from 96-well plates directly onto fiberglass filtermats and counted in the Wallac 1205 flat-bed scintillation counter (BetaPlate). The assay was validated by comparison with a traditional total collagen assay. The resulting assay provides a rapid one-step method for quantifying collagen synthesis, which, unlike many collagen assays, does not require extensive dialysis or precipitation of proteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0736-6205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1010-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11355336-Carbon Radioisotopes,
pubmed-meshheading:11355336-Cells, Cultured,
pubmed-meshheading:11355336-Collagen,
pubmed-meshheading:11355336-Collagenases,
pubmed-meshheading:11355336-Filtration,
pubmed-meshheading:11355336-Hydroxyproline,
pubmed-meshheading:11355336-Isotope Labeling,
pubmed-meshheading:11355336-Proline,
pubmed-meshheading:11355336-Scintillation Counting,
pubmed-meshheading:11355336-Tritium
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pubmed:year |
2001
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pubmed:articleTitle |
96-well plate-based method for total collagen analysis of cell cultures.
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pubmed:affiliation |
Rheumatology Research Unit, Box 194, E6, Addenbrookes Hospital NHS Trust, Hills Road, Cambridge CB2 2QQ, UK. saf25@medschl.cam.ac.uk
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pubmed:publicationType |
Comparative Study,
Research Support, Non-U.S. Gov't,
Technical Report
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