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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2001-5-16
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pubmed:abstractText |
The promoter for the Staphylococcus aureus capsule polysaccharide synthesis gene (cap1A) was cloned in front of the firefly luciferase gene for use in a cell extract S. aureus transcription-translation system. The assay is rapid, reproducible, and sensitive and has a lower background level than the radiolabeled amino acid incorporation translation assays. We present data evaluating a transcription inhibitor and a number of protein translation inhibitors in this system.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0066-4804
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1900-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11353649-Anti-Bacterial Agents,
pubmed-meshheading:11353649-Cloning, Molecular,
pubmed-meshheading:11353649-Drug Evaluation, Preclinical,
pubmed-meshheading:11353649-Genes, Reporter,
pubmed-meshheading:11353649-Luciferases,
pubmed-meshheading:11353649-Microbial Sensitivity Tests,
pubmed-meshheading:11353649-Plasmids,
pubmed-meshheading:11353649-Protein Biosynthesis,
pubmed-meshheading:11353649-Staphylococcus aureus,
pubmed-meshheading:11353649-Transcription, Genetic
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pubmed:year |
2001
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pubmed:articleTitle |
Staphylococcus aureus cell extract transcription-translation assay: firefly luciferase reporter system for evaluating protein translation inhibitors.
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pubmed:affiliation |
Pharmacia Corporation, Kalamazoo, Michigan 49007, USA.
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pubmed:publicationType |
Journal Article
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