Source:http://linkedlifedata.com/resource/pubmed/id/11353050
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2001-5-15
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| pubmed:abstractText |
Bcl-6 mRNA and protein are frequently expressed in the transformed counterparts of the germinal center B-cells, diffuse large B-cell lymphoma and follicular lymphoma, irrespective of the gene rearrangements. Most of the primary gastric lymphomas are thought to be of mucosa-associated lymphoid tissue (MALT) origin, and neither bcl-6 gene rearrangement nor protein expression is found in low-grade gastric lymphomas of the MALT type as in normal marginal zone cells. However, bcl-6 protein expression was identified in high-grade gastric lymphomas, suggesting its role in high-grade transformation. In this study, polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis for bcl-6 primer was performed in order to ascertain the molecular mechanisms of bcl-6 protein expression in primary gastric lymphomas. A total 31 cases of gastric lymphoma were classified into low-grade gastric lymphomas of MALT type (n = 13), high-grade gastric lymphomas of MALT type (n = 6) and gastric diffuse large B-cell lymphomas (n = 12). Bcl-6 mutations were observed in 11 of 13 (84.6%) low-grade gastric lymphomas of the MALT type and in 8 of 12 (66.7%) diffuse large B-cell gastric lymphomas. In 6 cases of the high-grade gastric lymphomas of the MALT type, both the low- and high-grade components demonstrated the same frequency (3/6, 50%) of mutations. The tissue obtained from the marginal zone of Peyer's patch by microdissection technique revealed no bcl-6 mutations by the PCR-SSCP analysis. These findings suggest that the acquisition process of bcl-6 mutations by the marginal zone cells may be involved in the lymphomagenesis of the stomach, but our data does not explain the reason why bcl-6 protein is expressed only in high-grade gastric lymphomas.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-6,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0893-3952
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
14
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
410-4
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11353050-DNA, Neoplasm,
pubmed-meshheading:11353050-DNA Mutational Analysis,
pubmed-meshheading:11353050-DNA-Binding Proteins,
pubmed-meshheading:11353050-Dissection,
pubmed-meshheading:11353050-Humans,
pubmed-meshheading:11353050-Lymphoma, B-Cell, Marginal Zone,
pubmed-meshheading:11353050-Lymphoma, Large B-Cell, Diffuse,
pubmed-meshheading:11353050-Micromanipulation,
pubmed-meshheading:11353050-Mutation,
pubmed-meshheading:11353050-Polymerase Chain Reaction,
pubmed-meshheading:11353050-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:11353050-Proto-Oncogene Proteins,
pubmed-meshheading:11353050-Proto-Oncogene Proteins c-bcl-6,
pubmed-meshheading:11353050-Stomach Neoplasms,
pubmed-meshheading:11353050-Transcription Factors
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| pubmed:year |
2001
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| pubmed:articleTitle |
Mutational analysis of the 5' noncoding region of the bcl-6 gene in primary gastric lymphomas.
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| pubmed:affiliation |
Department of Pathology, Dankook University College of Medicine, Cheonan, Chungnam, Korea. jaihyang@yahoo.co.kr
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| pubmed:publicationType |
Journal Article
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