|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5
|
|
pubmed:dateCreated |
2001-5-15
|
|
pubmed:abstractText |
The reticulocyte-type 15-lipoxygenase (15-LO-I) has been implicated in atherogenesis because of its capability of oxidizing low density lipoprotein. Therefore, we investigated the expression of the 15-LO-I gene in human umbilical vein endothelial cells (HUVEC). Nonactivated HUVEC did not exhibit detectable 15-LO-I mRNA. However, exposure of the cells to interleukin 4 (IL-4) induced the transcription of the 15-LO-I gene in a time- and concentration-dependent manner. Interestingly, this induction was not paralleled by a concomitant production of the functional 15-LO-I enzyme, as indicated by activity assays and immunoblotting. To gain more information about the mechanism of the induction process, we investigated IL-4-dependent activation of nuclear transcription factors for which binding sites were previously identified in the 5'-flanking region of the human 15-LO-I gene. Electrophoretic mobility shift assays revealed that IL-4 can activate signal transducer and activator of transcription 6, activator protein 2, GATA motif-binding transcription factor 1, nuclear factor 1, and SP-1 in HUVEC in a time- and concentration-dependent manner. Activation of these transcription factors was observed as early as 30 min after cytokine exposure. These data indicate that IL-4 upregulates the transcription of the 15-LO-I gene in human vascular endothelial cells, and this process may involve the activation of several nuclear transcription factors. The lack of active 15-LO-I protein in the presence of functional 15-LO-I mRNA suggests additional regulatory elements of 15-LO expression at posttranscriptional levels.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 15-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-2,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0022-2275
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
42
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
783-91
|
|
pubmed:dateRevised |
2009-11-3
|
|
pubmed:meshHeading |
pubmed-meshheading:11352986-Arachidonate 15-Lipoxygenase,
pubmed-meshheading:11352986-Arteriosclerosis,
pubmed-meshheading:11352986-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:11352986-Cells, Cultured,
pubmed-meshheading:11352986-Chromatography, High Pressure Liquid,
pubmed-meshheading:11352986-Cycloheximide,
pubmed-meshheading:11352986-DNA-Binding Proteins,
pubmed-meshheading:11352986-Endothelium, Vascular,
pubmed-meshheading:11352986-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:11352986-Gene Expression Regulation,
pubmed-meshheading:11352986-Humans,
pubmed-meshheading:11352986-Immunoblotting,
pubmed-meshheading:11352986-Interleukin-4,
pubmed-meshheading:11352986-NFI Transcription Factors,
pubmed-meshheading:11352986-Nuclear Proteins,
pubmed-meshheading:11352986-Protein Synthesis Inhibitors,
pubmed-meshheading:11352986-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11352986-STAT6 Transcription Factor,
pubmed-meshheading:11352986-Trans-Activators,
pubmed-meshheading:11352986-Transcription, Genetic,
pubmed-meshheading:11352986-Transcription Factor AP-2,
pubmed-meshheading:11352986-Transcription Factors,
pubmed-meshheading:11352986-Y-Box-Binding Protein 1
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
Interleukin 4 induces transcription of the 15-lipoxygenase I gene in human endothelial cells.
|
|
pubmed:affiliation |
Department of Surgery, University of Kentucky Medical Center, Lexington, KY 40536, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|
