Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-5-15
pubmed:abstractText
Fibroblast migration involves complex mechanical interactions with the underlying substrate. Although tight substrate contact at focal adhesions has been studied for decades, the role of focal adhesions in force transduction remains unclear. To address this question, we have mapped traction stress generated by fibroblasts expressing green fluorescent protein (GFP)-zyxin. Surprisingly, the overall distribution of focal adhesions only partially resembles the distribution of traction stress. In addition, detailed analysis reveals that the faint, small adhesions near the leading edge transmit strong propulsive tractions, whereas large, bright, mature focal adhesions exert weaker forces. This inverse relationship is unique to the leading edge of motile cells, and is not observed in the trailing edge or in stationary cells. Furthermore, time-lapse analysis indicates that traction forces decrease soon after the appearance of focal adhesions, whereas the size and zyxin concentration increase. As focal adhesions mature, changes in structure, protein content, or phosphorylation may cause the focal adhesion to change its function from the transmission of strong propulsive forces, to a passive anchorage device for maintaining a spread cell morphology.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10096925, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10198048, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10209151, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10318759, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10320946, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10375527, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10477757, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10550057, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10712519, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10783236, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-10866943, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-11259288, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-1169157, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-3536951, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-6987736, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-7593197, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-8182135, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-8608589, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-8939572, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9009194, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9082989, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-932106, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9391082, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9394617, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9660872, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9695809, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9739149, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9751904, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9751905, http://linkedlifedata.com/resource/pubmed/commentcorrection/11352946-9818165
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9525
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
881-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Nascent focal adhesions are responsible for the generation of strong propulsive forces in migrating fibroblasts.
pubmed:affiliation
Department of Physiology, University of Massachusetts Medical School, 377 Plantation Street, Worcester, MA 01605, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.