| pubmed-article:11352645 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11352645 | lifeskim:mentions | umls-concept:C0085080 | lld:lifeskim |
| pubmed-article:11352645 | lifeskim:mentions | umls-concept:C0164209 | lld:lifeskim |
| pubmed-article:11352645 | lifeskim:mentions | umls-concept:C0038585 | lld:lifeskim |
| pubmed-article:11352645 | lifeskim:mentions | umls-concept:C0243071 | lld:lifeskim |
| pubmed-article:11352645 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:11352645 | pubmed:dateCreated | 2001-5-15 | lld:pubmed |
| pubmed-article:11352645 | pubmed:abstractText | The internalization of [3H]propionyl[Met(O2)11]SP(7-11) which binds one binding site and of [3H][Pro9]SP which binds the two binding sites associated with the NK-1 receptor has been examined in CHO cells. The quantity of [3H][Pro9]SP measured inside the cytoplasm in kinetic experiments is fully temperature-dependent. In contrast, [3H]propionyl[Met(O2)11]SP(7-11) internalization reaches the same extent whatever the temperature, although the rate slowed down with lower temperature. The extent of internalization of [3H][Pro(9)]SP relative to the total specific bound is biphasic, when the extent of internalization of [3H]propionyl[Met(O2)11]SP(7-11) remains constant. For [3H][Pro9]SP, a high-affinity high-yield component inhibited in the presence of propionyl[Met(O2)11]SP(7-11) and a low-affinity low-yield component in the internalization process could be determined. Saturation studies show that [3H][Pro9]SP-binding parameters are insensitive to both phenylarsine oxide and monensin treatment, whereas [3H]propionyl[Met(O2)11]SP(7-11) maximal binding is decreased in both cases. Altogether, these data suggest that the two radiolabeled peptides should not follow the same internalization pathway. | lld:pubmed |
| pubmed-article:11352645 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11352645 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11352645 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11352645 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:11352645 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:11352645 | pubmed:author | pubmed-author:LavielleSS | lld:pubmed |
| pubmed-article:11352645 | pubmed:author | pubmed-author:SagaiTT | lld:pubmed |
| pubmed-article:11352645 | pubmed:copyrightInfo | Copyright 2001 Academic Press. | lld:pubmed |
| pubmed-article:11352645 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11352645 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:11352645 | pubmed:volume | 282 | lld:pubmed |
| pubmed-article:11352645 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11352645 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11352645 | pubmed:pagination | 958-64 | lld:pubmed |
| pubmed-article:11352645 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:meshHeading | pubmed-meshheading:11352645... | lld:pubmed |
| pubmed-article:11352645 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11352645 | pubmed:articleTitle | Internalization of [3H]substance P analogues in NK-1 receptor transfected CHO cells. | lld:pubmed |
| pubmed-article:11352645 | pubmed:affiliation | Unité Mixte de Recherches CNRS 7613, Chimie Organique Biologique, Université Pierre & Marie Curie, Aile 44-45, Bo $$;ite courrier 182, 4 place Jussieu, Paris cedex 05, 75252, France. sagan@ccr.jussieu.fr | lld:pubmed |
| pubmed-article:11352645 | pubmed:publicationType | Journal Article | lld:pubmed |
