rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5-6
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pubmed:dateCreated |
2001-5-11
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pubmed:abstractText |
We studied the activation of cab gene expression by phytochrome-induced intercellular signalling and report insights into the mechanism of induction and outspread of a plant internal signal. By micro-beam irradiation techniques and use of a photon-imaging charge-coupled device (CCD) camera system we monitored cab::luciferase reporter gene expression in cotyledons of transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants. We found that (i) the photoreceptor triggering intercellular signalling and reporter-gene expression is type II but not type I phytochrome, (ii) phytochrome in its far-red-absorbing (Pfr) form is necessary for the induction but not for the outspread of the signalling, (iii) red/far-red reversibility is restricted to the red-irradiated cells, and (iv) the phytochrome-induced signal spreads rapidly throughout the cotyledon and reaches its target cells within minutes.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
|
pubmed:issn |
0032-0935
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
212
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
759-64
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11346949-Arabidopsis Proteins,
pubmed-meshheading:11346949-Carrier Proteins,
pubmed-meshheading:11346949-Cotyledon,
pubmed-meshheading:11346949-Gene Expression Regulation, Plant,
pubmed-meshheading:11346949-Genes, Reporter,
pubmed-meshheading:11346949-Infrared Rays,
pubmed-meshheading:11346949-Kinetics,
pubmed-meshheading:11346949-Light,
pubmed-meshheading:11346949-Light-Harvesting Protein Complexes,
pubmed-meshheading:11346949-Luciferases,
pubmed-meshheading:11346949-Photoreceptor Cells,
pubmed-meshheading:11346949-Phytochrome,
pubmed-meshheading:11346949-Plants, Genetically Modified,
pubmed-meshheading:11346949-Plants, Toxic,
pubmed-meshheading:11346949-Recombinant Fusion Proteins,
pubmed-meshheading:11346949-Signal Transduction,
pubmed-meshheading:11346949-Tobacco
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pubmed:year |
2001
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pubmed:articleTitle |
Evidence for type II phytochrome-induced rapid signalling leading to cab::luciferase gene expression in tobacco cotyledons.
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pubmed:affiliation |
Hitachi Advanced Research Laboratory, Hatoyama, Saitama 350-0395, Japan. schuetz@mpiz-koeln.mpg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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