Source:http://linkedlifedata.com/resource/pubmed/id/11342546
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
29
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pubmed:dateCreated |
2001-7-16
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pubmed:abstractText |
In addition to their critical function in energy metabolism, mitochondria contain a permeability transition pore, which is regulated by adenine nucleotides. We investigated conditions required for ATP to induce a permeability transition in mammalian mitochondria. Mitochondrial swelling associated with mitochondria permeability transition (MPT) was initiated by adding succinate to a rat liver mitochondrial suspension containing alloxan, a diabetogenic agent. If alloxan was added immediately with or 5 min after adding succinate, MPT was strikingly decreased. MPT induced by alloxan was inhibited by EGTA and several agents causing thiol oxidation, suggesting that alloxan leads to permeability transition through a mechanism dependent on Ca(2+) uptake and sulfhydryl oxidation. Antimycin A and cyanide, inhibitors of electron transfer, carbonyl cyanide m-chlorophenylhydrazone, and oligomycin all inhibited MPT. During incubation with succinate, alloxan depleted ATP in mitochondria after an initial transient increase. However, in a mitochondrial suspension containing EGTA, ATP significantly increased in the presence of alloxan to a level greater than that of the control. These results suggest the involvement of energized transport of Ca(2+) in the MPT initiation. Addition of exogenous ATP, however, did not trigger MPT in the presence of alloxan and had no effect on MPT induced by alloxan. We conclude that alloxan-induced MPT requires mitochondrial energization, oxidation of protein thiols, and matrix ATP to promote energized uptake of Ca(2+).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
26942-6
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:11342546-Adenosine Triphosphate,
pubmed-meshheading:11342546-Alloxan,
pubmed-meshheading:11342546-Animals,
pubmed-meshheading:11342546-Calcium,
pubmed-meshheading:11342546-Cell Membrane Permeability,
pubmed-meshheading:11342546-Intracellular Membranes,
pubmed-meshheading:11342546-Male,
pubmed-meshheading:11342546-Mitochondria, Liver,
pubmed-meshheading:11342546-Oxidation-Reduction,
pubmed-meshheading:11342546-Rats,
pubmed-meshheading:11342546-Rats, Wistar,
pubmed-meshheading:11342546-Sulfhydryl Compounds
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pubmed:year |
2001
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pubmed:articleTitle |
Alloxan-induced mitochondrial permeability transition triggered by calcium, thiol oxidation, and matrix ATP.
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pubmed:affiliation |
Department of Biochemistry, Hokkaido College of Pharmacy, 7-1 Katsuraoka-cho, Otaru, Hokkaido 047-0264, Japan. ks51@hokuyakudai.ac.jp
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pubmed:publicationType |
Journal Article
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