Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:11342037rdf:typepubmed:Citationlld:pubmed
pubmed-article:11342037lifeskim:mentionsumls-concept:C0010762lld:lifeskim
pubmed-article:11342037lifeskim:mentionsumls-concept:C0205419lld:lifeskim
pubmed-article:11342037lifeskim:mentionsumls-concept:C0015219lld:lifeskim
pubmed-article:11342037lifeskim:mentionsumls-concept:C0439536lld:lifeskim
pubmed-article:11342037pubmed:issue1-2lld:pubmed
pubmed-article:11342037pubmed:dateCreated2001-5-8lld:pubmed
pubmed-article:11342037pubmed:abstractTextThe single mutant F87A of cytochrome P-450 BM-3 from Bacillus megaterium was engineered by rational evolution to achieve improved hydroxylation activity for medium chain length substrates (C8-C10). Rational evolution combines rational design and directed evolution to overcome the drawbacks of these methods when applied individually. Based on the X-ray structure of the enzyme, eight mutation sites (P25, V26, R47, Y51, S72, A74, L188, and M354) were identified by modeling. Sublibraries created by site-specific randomization mutagenesis of each single site were screened using a spectroscopic assay based on omega-p-nitrophenoxycarboxylic acids (pNCA). The mutants showing activity for shorter chain length substrates were combined, and these combi-libraries were screened again for mutants with even better catalytic properties. Using this approach, a P-450 BM-3 variant with five mutations (V26T, R47F, A74G, L188K, and F87A) that efficiently hydrolyzes 8-pNCA was obtained. The catalytic efficiency of this mutant towards omega-p-nitrophenoxydecanoic acid (10-pNCA) and omega-p-nitrophenoxydodecanoic acid (12-pNCA) is comparable to that of the wild-type P-450 BM-3.lld:pubmed
pubmed-article:11342037pubmed:languageenglld:pubmed
pubmed-article:11342037pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:citationSubsetIMlld:pubmed
pubmed-article:11342037pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:11342037pubmed:statusMEDLINElld:pubmed
pubmed-article:11342037pubmed:monthFeblld:pubmed
pubmed-article:11342037pubmed:issn0006-3002lld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:FischerMMlld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:SchmittJJlld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:SchmidR DRDlld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:PleissJJlld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:LiQ SQSlld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:Lutz-WahlSSlld:pubmed
pubmed-article:11342037pubmed:authorpubmed-author:SchwanebergUUlld:pubmed
pubmed-article:11342037pubmed:issnTypePrintlld:pubmed
pubmed-article:11342037pubmed:day9lld:pubmed
pubmed-article:11342037pubmed:volume1545lld:pubmed
pubmed-article:11342037pubmed:ownerNLMlld:pubmed
pubmed-article:11342037pubmed:authorsCompleteYlld:pubmed
pubmed-article:11342037pubmed:pagination114-21lld:pubmed
pubmed-article:11342037pubmed:dateRevised2008-11-21lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:meshHeadingpubmed-meshheading:11342037...lld:pubmed
pubmed-article:11342037pubmed:year2001lld:pubmed
pubmed-article:11342037pubmed:articleTitleRational evolution of a medium chain-specific cytochrome P-450 BM-3 variant.lld:pubmed
pubmed-article:11342037pubmed:affiliationInstitut für Technische Biochemie, Universität Stuttgart, Allmandring 31, D-70569, Stuttgart, Germany.lld:pubmed
pubmed-article:11342037pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:11342037pubmed:publicationTypeComparative Studylld:pubmed
pubmed-article:11342037pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11342037lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11342037lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11342037lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11342037lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:11342037lld:pubmed