Source:http://linkedlifedata.com/resource/pubmed/id/11340406
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2001-5-7
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pubmed:abstractText |
This study aimed to compare the effects of reduced glutathione (GSH) with those of S-(1,2-dicarboxyethyl)glutathione (DCE-GS) (in rabbits and humans), and different concentrations of the latter (in humans), on corneal endothelial permeability when added to solutions bathing the isolated cornea. Inulin/dextran permeability was determined from stromal- to endothelial-facing surfaces of de-epithelialized corneas. The bathing solution was modified Opeguard(R)-MA (MOMA), an ocular irrigating solution, to which either GSH or another intrinsic tripeptide, DCE-GS, was added. Paired corneas were used to compare either different combinations of GSH with DCE-GS (rabbit or human) or various concentrations of DCE-GS from 0.25 to 2.0 mM (human). Endothelial cyclic AMP levels were determined in cultured rabbit cells. MOMA alone resulted in approximately the same permeability as MOMA + 0.3 mM GSH while the use of 2 mM DCE-GS significantly reduced rabbit (40% maximum, p < 0.00001) and human (30% maximum, p < 0.01) corneal permeability. Human corneal endothelial permeability remained reduced through a range of concentrations of DCE-GS from 0.25 to 2.0 mM DCE-GS. Tissue-cultured rabbit corneal endothelium showed an increase in cyclic AMP after DCE-GS or GSH. DCE-GS potentially offers a viable alternative to GSH for inclusion in ocular irrigating or corneal preservative solutions since it maintains human corneal endothelial permeability at a lower, stable value relative to non-DCE-GS-containing solutions.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Dextrans,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Inulin,
http://linkedlifedata.com/resource/pubmed/chemical/S-(1,2-dicarboxyethyl)glutathione
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pubmed:status |
MEDLINE
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pubmed:issn |
0030-3747
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
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pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
151-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11340406-Aged,
pubmed-meshheading:11340406-Animals,
pubmed-meshheading:11340406-Cells, Cultured,
pubmed-meshheading:11340406-Cyclic AMP,
pubmed-meshheading:11340406-Dextrans,
pubmed-meshheading:11340406-Dose-Response Relationship, Drug,
pubmed-meshheading:11340406-Endothelium, Corneal,
pubmed-meshheading:11340406-Glutathione,
pubmed-meshheading:11340406-Humans,
pubmed-meshheading:11340406-Inulin,
pubmed-meshheading:11340406-Permeability,
pubmed-meshheading:11340406-Rabbits
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pubmed:articleTitle |
Human and rabbit corneal endothelial permeability after different chemical forms of glutathione.
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pubmed:affiliation |
Department of Ophthalmology, Medical College of Georgia, Augusta, Georgia 30912-3400, USA. kgreen@mail.mcg.edu
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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