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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2001-5-4
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pubmed:abstractText |
We analyzed the tissue distribution of apelin mRNA in rats by a quantitative reverse transcription-polymerase chain reaction and that of immunoreactive apelin (ir-apelin) by an enzyme immunoassay (EIA) using a monoclonal antibody. The expression levels of apelin mRNA and ir-apelin seemed to be consistent among tissues: they were highly expressed in the lung and mammary gland. By the combination of gel filtration and EIA, we found that the molecular forms of apelin differ among respective tissues: apelin molecules with sizes close to apelin-36 (long forms) were major components in the lung, testis, and uterus, but both long and short (whose sizes were close to [<Glu(65)]apelin-13) forms were detected in the mammary gland. In Scatchard analyses, the radioiodinated apelin-36 analogue bound to the receptor, APJ, with high affinity. In competitive binding assays, apelin-36 and apelin-19 far more efficiently inhibited the binding of the labeled apelin-36 analogue with APJ than [<Glu(65)]apelin-13. In analyses for the dissociation of apelin from APJ, unlabeled apelin-36 replaced more rapidly the labeled apelin-36 analogue bound with APJ than [<Glu(65)]apelin-13. Our results demonstrate that the long and short forms of apelin differently interact with APJ.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apln protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Aplnr protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0006-3002
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pubmed:author |
pubmed-author:FujiiRR,
pubmed-author:FujinoMM,
pubmed-author:FukusumiSS,
pubmed-author:HabataYY,
pubmed-author:HinumaSS,
pubmed-author:HosoyaMM,
pubmed-author:KawamataYY,
pubmed-author:KitadaCC,
pubmed-author:NEELH BHB,
pubmed-author:NishimuraOO,
pubmed-author:NishizawaNN
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
1538
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-71
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:11336787-Amino Acid Sequence,
pubmed-meshheading:11336787-Animals,
pubmed-meshheading:11336787-Carrier Proteins,
pubmed-meshheading:11336787-Chromatography, Gel,
pubmed-meshheading:11336787-Female,
pubmed-meshheading:11336787-Immunoenzyme Techniques,
pubmed-meshheading:11336787-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:11336787-Lung,
pubmed-meshheading:11336787-Male,
pubmed-meshheading:11336787-Mammary Glands, Animal,
pubmed-meshheading:11336787-Molecular Sequence Data,
pubmed-meshheading:11336787-Molecular Weight,
pubmed-meshheading:11336787-RNA, Messenger,
pubmed-meshheading:11336787-Rats,
pubmed-meshheading:11336787-Rats, Wistar,
pubmed-meshheading:11336787-Receptors, Dopamine D2,
pubmed-meshheading:11336787-Receptors, G-Protein-Coupled,
pubmed-meshheading:11336787-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11336787-Testis,
pubmed-meshheading:11336787-Uterus
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pubmed:year |
2001
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pubmed:articleTitle |
Molecular properties of apelin: tissue distribution and receptor binding.
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pubmed:affiliation |
Discovery Research Laboratories 1, Pharmaceutical Discovery Research Division, Takeda Chemical Industries, Ltd., Wadai 10, Tsukuba, 300-4293, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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