Linked Life Data

11336442

Source:http://linkedlifedata.com/resource/pubmed/id/11336442

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-5-4
pubmed:abstractText
Spinal cord injury (SCI) results in loss of locomotor function and development of abnormal chronic pain syndromes (mechanical allodynia, thermal hyperalgesia). Following injury, secondary mechanisms including release of excitatory amino acids, inflammation and lipid peroxidation damage neural cells through release of cytotoxic free radicals. We hypothesized that selective inhibition of cyclooxygenase-2 (COX-2), an inducible inflammatory mediator, would decrease tissue damage and subsequently reduce locomotor deficits and development of chronic central pain syndromes after injury. Fifteen minutes prior to receiving T13 spinal segment spinal cord contusion injury, 200-225-g male Sprague-Dawley rats received either vehicle (0.5 ml 1:1 v/v DMSO/saline, i.p., n = 20) or the selective COX-2 inhibitor NS-398 (5 mg/kg in DMSO/saline v/v, i.p., n = 20). Locomotor function via the BBB scale, and nociceptive behaviors measured by paw withdrawals to von Frey filaments and radiant heat stimuli were tested for 4 weeks postinjury. Histological examination and volumetric analysis of spinal cord tissue were performed concomitantly. Spinally contused animals receiving NS-398 demonstrated significantly (p < 0.05) reduced locomotor alteration and reductions in both fore- and hindlimb mechanical allodynia and thermal hyperalgesia when compared to vehicle controls. Histological examination of spinal segments at the lesion segment demonstrated reduced lesion extent and increased viable tissue when compared to vehicle controls. Prostaglandin E2 levels were significantly lowered in NS-398-treated but not vehicle-treated animals 12 h after injury. These results support the role of COX-2 in reducing pathological and behavioral deficits after spinal cord injury.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0897-7151
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
409-23
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11336442-Animals, pubmed-meshheading:11336442-Behavior, Animal, pubmed-meshheading:11336442-Contusions, pubmed-meshheading:11336442-Cyclooxygenase 2, pubmed-meshheading:11336442-Cyclooxygenase 2 Inhibitors, pubmed-meshheading:11336442-Cyclooxygenase Inhibitors, pubmed-meshheading:11336442-Dinoprostone, pubmed-meshheading:11336442-Hot Temperature, pubmed-meshheading:11336442-Hypesthesia, pubmed-meshheading:11336442-Immunohistochemistry, pubmed-meshheading:11336442-Isoenzymes, pubmed-meshheading:11336442-Male, pubmed-meshheading:11336442-Motor Activity, pubmed-meshheading:11336442-Nitrobenzenes, pubmed-meshheading:11336442-Physical Stimulation, pubmed-meshheading:11336442-Prostaglandin-Endoperoxide Synthases, pubmed-meshheading:11336442-Rats, pubmed-meshheading:11336442-Rats, Sprague-Dawley, pubmed-meshheading:11336442-Spinal Cord Injuries, pubmed-meshheading:11336442-Sulfonamides
pubmed:year
2001
pubmed:articleTitle
Reduction of pathological and behavioral deficits following spinal cord contusion injury with the selective cyclooxygenase-2 inhibitor NS-398.
pubmed:affiliation
Department of Anatomy and Neurosciences and Marine Biomedical Institute, University of Texas Medical Branch, Galveston 77555-1069, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't