Linked Life Data

11336066

Source:http://linkedlifedata.com/resource/pubmed/id/11336066

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-5-1
pubmed:abstractText
Streptomyces cinnamonensis A-10 is a monensin \ producing strain. pHZ1252 is an E. coli-Streptomyces shuttle vector for expressing VHb, in which vhb structural gene is controlled by thiostrepton-inducible promoter PtipA. pHZ1252 was unstable in S. cinnamonensis, occurring deletion recombination. The deletion DNA fragment of pHZ1252 included not only part of E. coli plasmid but also vhb gene. However, plasmid pHZ1252 isolated from S. avermitilis transformants, which lost part of E. coli plasmid but still contained vhb gene and PtipA was stable in S. cinnamonensis and expressed VHb with biological activity after thiostrepton induction. Shake flask fermentation experiments showed that the presence of VHb in S. cinnamonensis efficiently enhanced cell growth and improved antibiotic production under aeration-poor conditions.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1000-3061
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
24-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[The effects of Vitreoscilla hemoglobin expression on growth and antibiotic production in Streptomyces cinnamonensis].
pubmed:affiliation
College of Biological Science, China Agricultural University, Beijing 100094, China. dong.wen@263.net
pubmed:publicationType
Journal Article, English Abstract