Linked Life Data

11330859

Source:http://linkedlifedata.com/resource/pubmed/id/11330859

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-5-1
pubmed:abstractText
Palatal clefting is often associated with premature fusion of cranial sutures in human craniosynostosis syndromes, many of which are characterised by mutations affecting the fibroblast growth factor receptor (FGFR) gene family. In palatal fusion, epithelio-mesenchymal transition (EMT) contributes to the dispersion of the midline epithelial seam. EMT has also been observed in neoplastic epithelial cells in relation to the acquisition of malignant characteristics where morphological changes are accompanied by rapid switching in the expression of fgfr2 from the epithelial type (kgfr) to the mesenchymal type (bek). The twist gene codes for a basic helix-loop-helix transcription factor putatively involved in regulation of transcription of fgfr2. Mutations in the TWIST gene have been described as being responsible for the Saethre-Chotzen syndrome, an autosomal dominant craniosynostosis associated with cleft palate as well as other disturbances of the facial skeleton. In this study we have analysed the distribution of twist transcripts during rat palatogenesis in vivo from 14.5 to 17.5 days post coitum by in situ hybridisation with digoxygenin-labelled ssDNA probes. twist transcripts were found to be concentrated in mesenchymal cells beneath the epithelium at the tip of the palatal shelves immediately prior to, and during fusion as well as in a localised epithelial area at the tip of the shelves prior to fusion, thereby implicating twist gene expression in the process of palatogenesis. This pattern of expression illuminates the disturbances of maxillary growth that occur in human craniosynostotic syndromes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0214-6282
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
397-404
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11330859-Amino Acid Sequence, pubmed-meshheading:11330859-Animals, pubmed-meshheading:11330859-Base Sequence, pubmed-meshheading:11330859-Gene Expression Regulation, Developmental, pubmed-meshheading:11330859-Helix-Loop-Helix Motifs, pubmed-meshheading:11330859-In Situ Hybridization, pubmed-meshheading:11330859-Male, pubmed-meshheading:11330859-Molecular Sequence Data, pubmed-meshheading:11330859-Nuclear Proteins, pubmed-meshheading:11330859-Palate, pubmed-meshheading:11330859-Polymerase Chain Reaction, pubmed-meshheading:11330859-Protein Structure, Tertiary, pubmed-meshheading:11330859-Rats, pubmed-meshheading:11330859-Rats, Sprague-Dawley, pubmed-meshheading:11330859-Receptor, Fibroblast Growth Factor, Type 2, pubmed-meshheading:11330859-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:11330859-Receptors, Fibroblast Growth Factor, pubmed-meshheading:11330859-Sequence Alignment, pubmed-meshheading:11330859-Transcription Factors, pubmed-meshheading:11330859-Twist Transcription Factor
pubmed:year
2001
pubmed:articleTitle
R-twist gene expression during rat palatogenesis.
pubmed:affiliation
Department of Paediatric Dentistry, Eastman Dental Institute for Oral Health Care Sciences, University College London, England. A.Blochzupan@eastman.ucl.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't